Comparison between two different experimental models of osteoarthritis in rabbits. Intra-articular collagenase injection and anterior cruciate ligament transection
| Autor: | Paulo Henrique de Affonseca Jardim, Larissa Correa Hermeto, Rafael de Rossi, Luis A. Justulin, Jaqueline C. Rinaldi, Aureo Evangelista Santana |
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| Přispěvatelé: | Universidade Federal de Mato Grosso do Sul (UFMS), Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cartilage Articular Male RD1-811 Knee Joint Anterior cruciate ligament Stifle joint Osteoarthritis Injections Intra-Articular 03 medical and health sciences Random Allocation 0302 clinical medicine Intra articular medicine Animals Collagenases Anterior Cruciate Ligament 030203 arthritis & rheumatology Ligaments Collagenase injection business.industry Cartilage Anatomy medicine.disease musculoskeletal system Disease Models Animal 030104 developmental biology medicine.anatomical_structure Collagenase Surgery Rabbits Joint Diseases business medicine.drug |
| Zdroj: | Acta Cirúrgica Brasileira v.31 n.9 2016 Acta Cirúrgica Brasileira Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC Acta Cirurgica Brasileira, Volume: 31, Issue: 9, Pages: 602-607, Published: SEP 2016 Acta Cirurgica Brasileira, Vol 31, Iss 9, Pp 602-607 (2016) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0102-8650 |
| Popis: | Made available in DSpace on 2018-12-11T17:29:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-09-01. Added 1 bitstream(s) on 2019-10-09T18:32:40Z : No. of bitstreams: 1 S0102-86502016000900602.pdf: 659754 bytes, checksum: 1e6171a2115bc2adf8f43f3328180755 (MD5) PURPOSE: To compare two different experimental models of osteoarthritis in rabbits: intra-articular collagenase injection and anterior cruciate ligament transection. METHODS: Ten adult rabbits were randomly divided in two groups: COLL (collagenase group) and ACLT (anterior cruciate ligament transection). The COLL group was treated with 0.5 ml collagenase solution (2mg collagenase/0.5 ml sterile PBS), and the ACTL group was subjected to anterior cruciate ligament. After six and twelve weeks, respectively, the animals in the COLL and ACTL groups were euthanized. The gross appearance and histological examinations conducted in the cartilage articular surface was blindly scored according to the criteria developed by Yoshimi et al. (1994) and Mankin et al. (1971), respectively. RESULTS: The gross morphologic observation, macroscopic score and histological examinations have demonstrated that the ACTL group presented the highest scores, and lesions more severe than those in the COLL group. CONCLUSIONS: Both methods, anterior cruciate ligament transection and collagenase, applied to the stifle joint of the rabbits have effectively induced degenerative changes in the cartilage tissue, through statistically significant analysis (p≤0.05). The ACTL method has presented more severe lesions. Veterinary Sciences Veterinary Medicine and Animal Science School Universidade Federal do Mato Grosso do Sul (UFMS) Anesthesiology and Surgery Sector Department of Veterinary Medicine Veterinary Medicine and Animal Science School UFMS Department of Veterinary Medicine School of Veterinary Medicine and Animal Science School UFMS Department of Veterinary Clinical Medicine and Surgery Agrarian and Veterinary Sciences School Universidade Estadual de S�o Paulo (UNESP) Institute of Biosciences Department of Morphology Extracellular Matrix Laboratory UNESP Department of Veterinary Clinical Medicine and Surgery Agrarian and Veterinary Sciences School Universidade Estadual de S�o Paulo (UNESP) Institute of Biosciences Department of Morphology Extracellular Matrix Laboratory UNESP |
| Databáze: | OpenAIRE |
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