In vitro transcription of wound tumor virus RNA by virion-associated RNA transcriptase
| Autor: | Amiya K. Banerjee, Dennis P. Rhodes, Judith A. Lesnaw, Roderick MacLeod, D.V.R. Reddy, L.M. Black |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Manganese
Chymotrypsin Cell-Free System Transcription Genetic biology RNA-Directed DNA Polymerase RNA-dependent RNA polymerase RNA DNA-Directed RNA Polymerases Wound tumor virus biology.organism_classification Molecular biology In vitro Virus Plant Viruses Phosphoenolpyruvate Plant Tumors Virology biology.protein RNA Viral Magnesium Phosphoenolpyruvate Carboxykinase (GTP) Polymerase |
| Zdroj: | Virology. 80:356-361 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/s0042-6822(77)80011-1 |
| Popis: | The results of this communication indicate that wound tumor virus (WTV) has a virion-bound polymerase which can synthesize all 12 single-stranded RNA copies of the genome segments in vitro . Chymotrypsin treatment of the purified virus did not affect its transcriptase activity. Previous observations have shown that virus treated with chymotrypsin lacks the 131,000- and 96,000-dalton polypeptides. Since we have shown in the present study that the enzyme activity was retained following chymotrypsin treatment, we conclude that neither of these polypeptides are the polymerase. In addition, all cations tested, with the exception of Mg 2+ and Mn 2+ , did not enhance the enzyme activity. Phosphoenolpyruvate (PEP) together with PEP-kinase substantially increased in vitro transcription, and the transcribed products, when annealed with denatured WTV genome RNA, gave an electrophoretic profile identical to that of the native viral genome. |
| Databáze: | OpenAIRE |
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