Effect of Maternal Obesity on Fetal Growth and Expression of Placental Fatty Acid Transporters
Autor: | Yi Li, Dan Zhang, Chuan Lai Hu, Hai Qing Wang, Kui Ye, Han Lin Lai, Li Li |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Male medicine.medical_specialty placenta Endocrinology Diabetes and Metabolism CD36 intrauterine growth Diet High-Fat Fetal Development Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Endocrinology Western blot nutrient transport Pregnancy Placenta Internal medicine medicine Animals Obesity chemistry.chemical_classification Fetus 030219 obstetrics & reproductive medicine biology medicine.diagnostic_test business.industry Fatty Acids Fatty acid Maternal Nutritional Physiological Phenomena medicine.disease Fatty Acid Transport Proteins Rats Pregnancy Complications 030104 developmental biology medicine.anatomical_structure high-fat diet chemistry Pediatrics Perinatology and Child Health biology.protein Gestation Original Article Female business |
Zdroj: | Journal of Clinical Research in Pediatric Endocrinology |
ISSN: | 1308-5735 1308-5727 |
Popis: | Objective: To explore the effects of maternal high-fat (HF) diet-induced obesity on fetal growth and the expression of placental nutrient transporters. Methods: Maternal obesity was established in rats by 8 weeks of pre-pregnancy fed HF diet, while rats in the control group were fed normal (CON) diet. Diet-induced obesity (DIO) rats and diet-induced obesity-resistant (DIR) rats were selected according to body weight gain over this period. After copulation, the CON rats were divided into two groups: switched to HF diet (CON-HF group) or maintained on the CON diet (CON-CON group). The DIO rats and DIR rats were maintained on the HF diet throughout pregnancy. Pregnant rats were euthanized at day 21 gestation, fetal and placental weights were recorded, and placental tissue was collected. Reverse transcription-polymerase chain reaction was used to determine mRNA expression of placental nutrient transporters. Protein expression was determined by Western blot. Results: Average fetal weight of DIO dams was reduced by 6.9%, and the placentas of CON-HF and DIO dams were significantly heavier than the placentas of CON-CON and DIR dams at day 21 of gestation (p |
Databáze: | OpenAIRE |
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