Synergistic role of nanoceria on the ability of tobacco smoke to induce carcinogenic hallmarks in lung epithelial cells
| Autor: | Jordi Bach, Ricard Marcos, Laura Rubio, Alba Hernández |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Carcinogenesis
Cell Biomedical Engineering Medicine (miscellaneous) Bioengineering 02 engineering and technology 010501 environmental sciences Development Pharmacology medicine.disease_cause 01 natural sciences Cell Movement Smoke Tobacco Gene expression Biomarkers Tumor medicine Humans General Materials Science Cell Shape Lung Carcinogen Cell Proliferation 0105 earth and related environmental sciences Cell growth Chemistry Cell Differentiation Epithelial Cells Cell migration Cerium 021001 nanoscience & nanotechnology Phenotype In vitro Cell Transformation Neoplastic medicine.anatomical_structure Matrix Metalloproteinase 9 Cancer research Nanoparticles 0210 nano-technology |
| Zdroj: | Nanomedicine. 12:2623-2635 |
| ISSN: | 1748-6963 1743-5889 |
| Popis: | Aim: Since controversial results have been obtained in studies dealing with nanoceria usefulness in biomedical applications, the transforming effects of long-term exposure to nanoceria in lung epithelial cells, alone or together with cigarette smoke condensate (CSC), were evaluated. Materials & methods: In vitro cell transformation techniques were used to study several hallmarks of carcinogenesis. Morphology, cell proliferation, gene expression, cell migration, anchorage-independent cell growth and cell secretome were analyzed. Results & conclusion: Data evidence no transforming ability of nanoceria, but support a synergistic role on CSC's transforming ability. A more noticeable spindle-like phenotype, increased proliferation rate, higher degree of differentiation status dysregulation, higher migration capacity, increased anchorage-independent cell growth and higher levels of MMP-9 and cell growth promoting capability, were observed. In addition, nanoceria co-exposure exacerbates the expression of FRA-1. |
| Databáze: | OpenAIRE |
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