A simple electrochemical detection of atorvastatin based on disposable screen-printed carbon electrodes modified by molecularly imprinted polymer: Experiment and simulation
| Autor: | Patrícia Rebelo, João G. Pacheco, Iuliia V. Voroshylova, André Melo, M. Natália D.S. Cordeiro, Cristina Delerue-Matos |
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| Přispěvatelé: | Repositório Científico do Instituto Politécnico do Porto |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Electropolymerisation
Molecularly imprinted polymer Statins Electrochemical Techniques Biochemistry Carbon Analytical Chemistry Molecular Imprinting Molecularly Imprinted Polymers Electrochemical sensor Limit of Detection Atorvastatin Environmental Chemistry Electrodes Environmental analysis Spectroscopy |
| Popis: | Atorvastatin (ATV) is a statin member consumed in high quantities worldwide. In response to that, the occurrence of ATV in environmental waters has become a reality, highlighting the need of rapid and sensitive analytical devices for its monitoring. In this work, the first electrochemical molecularly imprinted polymer (MIP) sensor for the detection of ATV in water samples is presented. Computational studies were conducted based on quantum mechanical (QM) calculations and molecular dynamics (MD) simulations for rational selection of a suitable functional monomer and to study in detail the template-monomer interaction, respectively. The sensor was prepared by electropolymerisation of the selected 4-aminobenzoic acid (ABA) monomer with ATV, acting as template, on screen printed carbon electrode (SPCE). Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques were applied to characterise the modified electrode surfaces. The quantitative measurements were carried out with differential pulse voltammetry (DPV) in 0.1 M phosphate buffer (pH = 7). After investigation and optimisation of important experimental parameters, a linear working range down to 0.05 μmol L−1 was determined with a correlation coefficient of 0.9996 and a limit of detection (LOD) as low as 0.049 μmol L−1 (S/N = 3). High sensitivity and selectivity of the prepared sensor were demonstrated with the ability to recognise ATV molecules over its closer structural analogues. Moreover, the sensor was quickly and successfully applied in spiked water samples, proving its potential for future on-site monitoring of ATV in environmental waters. Patrícia Rebelo (SFRH/BD/132384/2017) and João Pacheco (SFRH/BPD/101419/2014) are grateful to Fundação para a Ciência e a Tecnologia (FCT) and European Union (EU) for their grants, financed by POPH-QREN-Tipologia 4.1-Formação Avançada, funded by Fundo Social Europeu (FSE) and Ministério da Ciência, Tecnologia e Ensino Superior (MCTES). The work was supported by the project Farmasense(39957) that was funded by Sistema de Incentivos à Investigação e Desenvolvimento Tecnológico de Portugal 2020, through the Programa Operacional do Norte (NORTE 2020) and the Fundo Europeu de Desenvolvimento Regional (FEDER). This work was further supported by UID/QUI/50006/2020 (LAQV-REQUIMTE) with funding from FCT/MCTES through national funds. The authors would like to thank the EU and FCT/UEFISCDI/FORMAS for funding, in the frame of the collaborative international consortium REWATER financed under the ERA-NET Cofund WaterWorks 2015 Call. This ERA-NET is an integral part of the 2016 Joint Activities developed by the Water Challenges for a Changing World Joint Programme Initiative (Water JPI). |
| Databáze: | OpenAIRE |
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