Inotodiol From Inonotus obliquus Chaga Mushroom Induces Atypical Maturation in Dendritic Cells
| Autor: | Perry Ayn Mayson A. Maza, Ji-Hyun Lee, Yong-Su Kim, Gyu-Min Sun, Youn-Joo Sung, Ludmila P. Ponomarenko, Valentine A. Stonik, Minsook Ryu, Jong-Young Kwak |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
T cell Immunology inotodiol Bone Marrow Cells IκB kinase CD8-Positive T-Lymphocytes Wortmannin chemistry.chemical_compound Lanosterol Mice Downregulation and upregulation medicine Immunology and Allergy Animals dendritic cells Phosphorylation Cells Cultured Original Research Cell Proliferation CD86 Inotodiol AKT1 Mice Inbred BALB C CD40 biology Molecular Structure IL-2 Cell Differentiation Cell biology Inonotus Mice Inbred C57BL medicine.anatomical_structure RAW 264.7 Cells chemistry biology.protein Chaga mushroom Cytokines lcsh:RC581-607 Agaricales Proto-Oncogene Proteins c-akt CD8 atypical maturation |
| Zdroj: | Frontiers in Immunology Frontiers in Immunology, Vol 12 (2021) |
| ISSN: | 1664-3224 |
| Popis: | Dendritic cells (DCs) have the ability to stimulate naïve T cells that coordinate subsequent adaptive response toward an inflammatory response or tolerance depending on the DC differentiation level. Inotodiol, a lanostane triterpenoid found in Inonotus obliquus (wild Chaga mushroom), is a natural compound with a wide range of biological activities. In this study, we investigated whether inotodiol promotes the maturation of bone marrow-derived DCs (BMDCs) and inotodiol-treated BMDCs induce T cell activation. Inotodiol increased the expression of surface maturation markers, including MHC-I, MHC-II, CD86, and CD40, on BMDCs without affecting the production of various cytokines, including TNF-α and IL-12p40 in these cells. T cells primed with inotodiol-treated BMDCs proliferated and produced IL-2, without producing other cytokines, including IL-12p40 and IFN-γ. Injection of inotodiol into mice induced maturation of splenic DCs and IL-2 production, and the administration of inotodiol and inotodiol-treated BMDCs induced the proliferation of adoptively transferred CD8+ T cells in vivo. The phosphatidylinositol-3-kinase inhibitor wortmannin abrogated the upregulation of Akt phosphorylation and CD86 and MHC-II expression induced by inotodiol. However, inotodiol failed to induce phosphorylation of the IκB kinase and degradation of IκB-α, and increased expression of CD86 induced by inotodiol was not blocked by an IκB kinase inhibitor. These results suggest that inotodiol induces a characteristic type of maturation in DCs through phosphatidylinositol-3-kinase activation independent of NF-κB, and inotodiol-treated DCs enhance T cell proliferation and IL-2 secretion. |
| Databáze: | OpenAIRE |
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