Functional Expression of Large-Conductance Calcium-Activated Potassium Channels in Human Endometrium: A Novel Mechanism Involved in Endometrial Receptivity and Embryo Implantation
| Autor: | Yong-Chao Lu, Ai-Xia Liu, He-Feng Huang, Fan Qu, Ya-Jing Tan, Li-Bo Zou, Dan Zhang, Guo-Lian Ding, Ting-Ting Wang, Ping-Ping Lv, Ye Meng, Run-Ju Zhang, Jian-Zhong Sheng |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
medicine.medical_specialty Small interfering RNA Biopsy Endocrinology Diabetes and Metabolism Primary Cell Culture Clinical Biochemistry Biology Biochemistry Endometrium Mice Endocrinology Western blot In vivo Internal medicine medicine Animals Humans Embryo Implantation Large-Conductance Calcium-Activated Potassium Channels RNA Small Interfering Cells Cultured Menstrual Cycle medicine.diagnostic_test Biochemistry (medical) Embryo Transfection Calcium-activated potassium channel Potassium channel Gene Expression Regulation Cell culture Female |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 97:543-553 |
| ISSN: | 1945-7197 0021-972X |
| Popis: | Large-conductance calcium-activated potassium channels (BK(Ca) channels) mediate physiological processes in nonexcitable cells.The aim of the study was to determine BK(Ca) channel expression in human endometrium and its role in endometrial receptivity and embryo implantation.BK(Ca) channel expression in human endometrium is described at different phases of the menstrual cycle using quantitative real time-PCR and Western blot techniques. Their effects on embryo implantation were examined using JAr spheroid attachment assays and in vivo mouse model. We examined their effects on endometrial receptivity factors, nuclear factor-κB (NF-κB) activity using quantitative real time-PCR, Western blot, and EMSA analyses. Changes in electrophysiological properties and cytosolic free Ca(2+) were measured in endometrial cells with or without specific BK(Ca) blocker or transfected with BK(Ca) small interfering RNA using patch-clamp and fluorescence analyses, respectively.BK(Ca) channels are expressed in human endometrial cells in a phase-related fashion during the menstrual cycle (proliferative, 0.20 ± 0.02, vs. mid-secretory, 0.72 ± 0.07; P0.01). Blocking BK(Ca) channel function or knockdown of endogenous BK(Ca) channel expression not only decreased JAr spheroid attachment rate and embryo implantation rate in mice but also significantly reduced the expression levels of endometrial receptive factors, including leukemia inhibitory factor, integrin β3, claudin-4, and DKK-1, in human endometrial cells. Blocking BK(Ca) channels also reduced BK(Ca)-regulated NF-κB activity, cytosolic Ca(2+) concentrations, and membrane potentials in human endometrial cells.These observations demonstrate that BK(Ca) channels: 1) are expressed in endometrial cells; 2) affect embryo implantation by mediating endometrial receptive factors; and 3) alter the activity of NF-κB and homeostasis of Ca(2+) in the human endometrial cells. |
| Databáze: | OpenAIRE |
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