Comparision of atomic force microscopy interaction forces between bacteria and silicon nitride substrata for three commonly used immobilization methods
| Autor: | Willem Norde, Joop de Vries, Ietse Stokroos, Henderina van der Mei, Henk J. Busscher, Rene J. B. Dijkstra, Virginia Vadillo-Rodríguez |
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| Přispěvatelé: | Man, Biomaterials and Microbes (MBM), Personalized Healthcare Technology (PHT) |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
binding
Microscope Materials science SURFACE Laboratorium voor Fysische chemie en Kolloïdkunde Nanotechnology dna Microscopy Atomic Force Applied Microbiology and Biotechnology Bacterial cell structure law.invention chemistry.chemical_compound Adsorption law Klebsiella BINDING Methods surface afm Sub-department of Water Resources Sectie Waterhuishouding adhesion forces Physical Chemistry and Colloid Science VLAG Ecology biology Bacteria Cell Membrane Silicon Compounds DNA biology.organism_classification Membrane Silicon nitride chemistry Nanometre Glutaraldehyde AFM ADHESION FORCES Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology, 70(9), 5441-5446 Applied and environmental microbiology, 70(9), 5441-5446. AMER SOC MICROBIOLOGY Applied and Environmental Microbiology 70 (2004) 9 |
| ISSN: | 1098-5336 0099-2240 5441-5446 |
| Popis: | Atomic force microscopy (AFM) has emerged as a powerful technique for mapping the surface morphology of biological specimens, including bacterial cells. Besides creating topographic images, AFM enables us to probe both physicochemical and mechanical properties of bacterial cell surfaces on a nanometer scale. For AFM, bacterial cells need to be firmly anchored to a substratum surface in order to withstand the friction forces from the silicon nitride tip. Different strategies for the immobilization of bacteria have been described in the literature. This paper compares AFM interaction forces obtained between Klebsiella terrigena and silicon nitride for three commonly used immobilization methods, i.e., mechanical trapping of bacteria in membrane filters, physical adsorption of negatively charged bacteria to a positively charged surface, and glutaraldehyde fixation of bacteria to the tip of the microscope. We have shown that different sample preparation techniques give rise to dissimilar interaction forces. Indeed, the physical adsorption of bacterial cells on modified substrata may promote structural rearrangements in bacterial cell surface structures, while glutaraldehyde treatment was shown to induce physicochemical and mechanical changes on bacterial cell surface properties. In general, mechanical trapping of single bacterial cells in filters appears to be the most reliable method for immobilization. |
| Databáze: | OpenAIRE |
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