Molecular epidemiology of hepatitis B and Delta virus strains that spread in the Mediterranean North East Coast of Tunisia
| Autor: | Frédéric Le Gal, Nabil Ben Mami, Amel Sadraoui, Azouz Msaddek, Imed Cheikh, Ségolène Brichler, Wael Mansour, Emmanuel Gordien, Henda Triki, Walid Hammami, Lamia Yacoubi |
|---|---|
| Přispěvatelé: | Université de Carthage - University of Carthage, Laboratoire de Virologie Clinique, Référence Régional OMS pour la Poliomyélite et la Rougeole - Laboratory of Clinical Virology, WHO Regional Reference Laboratory on Poliomyelitis and Measles, Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Service de bactériologie-Virologie-Hygiène [Avicenne], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris 13 (UP13)-Hôpital Avicenne [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Hôpital La Rabta [Tunis], Université de Tunis El Manar (UTM), Department of Gastroenterology, Tahar Maamouri Hospital, Nabeul, Tunisia, Department of Gastroenterology, Habib Bougatfa Hospital, Bizerte, Tunisia, This study was partially supported by the Tunisian Ministry for Scientific Research and Technology (LR11-IPT09). |
| Rok vydání: | 2015 |
| Předmět: |
Male
MESH: Sequence Analysis DNA HBsAg [SDV]Life Sciences [q-bio] viruses Prevalence Seroprevalence medicine.disease_cause MESH: Genotype MESH: Aged 80 and over MESH: Mediterranean Region 0302 clinical medicine MESH: Risk Factors Risk Factors MESH: Child Genotype HBV MESH: Genetic Variation MESH: Phylogeny Child Phylogeny MESH: Aged Aged 80 and over 0303 health sciences MESH: Middle Aged Mediterranean Region virus diseases Hepatitis B Middle Aged Viral Load Hepatitis D 3. Good health MESH: Hepatitis B virus Infectious Diseases MESH: Young Adult MESH: RNA Viral [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology RNA Viral 030211 gastroenterology & hepatology Female Hepatitis Delta Virus MESH: Tunisia MESH: Viral Load Viral load Adult Hepatitis B virus Tunisia Adolescent Genotypes Biology 03 medical and health sciences Young Adult HDV Virology medicine Humans MESH: Prevalence 030304 developmental biology Aged Retrospective Studies MESH: Adolescent Hepatitis MESH: Hepatitis D MESH: Humans MESH: Hepatitis B Molecular epidemiology Genetic Variation MESH: Adult MESH: Retrospective Studies [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology Sequence Analysis DNA biochemical phenomena metabolism and nutrition medicine.disease MESH: Hepatitis Delta Virus MESH: Male MESH: DNA Viral DNA Viral MESH: Female |
| Zdroj: | Journal of Clinical Virology Journal of Clinical Virology, Elsevier, 2015, 72, pp.126-132. ⟨10.1016/j.jcv.2015.10.002⟩ |
| ISSN: | 1873-5967 1386-6532 |
| DOI: | 10.1016/j.jcv.2015.10.002⟩ |
| Popis: | International audience; Background: Tunisia is classified as an area of middle endemic for hepatitis B virus (HBV) infection, however little is known about hepatitis Delta virus (HDV) infection. Objectives: This study aimed to address the prevalence of HDV infection, to identify possible risks factors, and to analyze the genetic diversity of HDV strains that are spreading in Tunisia. Study design: A retrospective large-scale study including 1615 HBsAg positive patients, native of the North East coast of Tunisia, recruited from Gastroenterology departments, was conducted. Demographic, epidemiological, ethnical, clinical and biological data were recorded. HBV and HDV serological analyses and DNA and RNA viral load quantification were performed. Genotyping of HBV and HDV strains was performed using nucleotide sequencing followed by phylogenetic analyses. Results: The study population included 819(50.7%) men and 796(49.3%) women; aged 12-90 years (mean age 41 + 13 years). A very low prevalence of HDV infection, 2% was observed. No risk factor, except a history of hospitalization for surgery was found. All HDV strains belonged to genotype 1, with a wide distribution within the HDV-1 group. They all share the African amino acid marker, a serine at position 202 of the large Delta protein. HBV genotypes were distributed as follows: HBV/D1 (56.8%), HBV/D7 (40.9%), and HBV/A2 (2.3%). Conclusion: Tunisia is a low endemic region for HDV infection, due to an efficient policy of HBV infection control. HDV-1 is the sole genotype found, with a high diversity within this group. Further studies are ongoing in order to better characterize and manage the HBV/HDV-infected patients according to the genetic variability of the viral strains. (C) 2015 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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