MALDI-TOF MS analysis of soluble PEG based multi-step synthetic reaction mixtures with automated detection of reaction failure
| Autor: | Jean-Louis Aubagnac, Neerja Yadav-Bhatnagar, Jean Martinez, Frédéric Lamaty, Patrice Ribière, Christine Enjalbal |
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| Přispěvatelé: | Laboratoire des Amino-acides Peptides et Protéines (LAPP), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Aventis Pharma, Aventis |
| Rok vydání: | 2005 |
| Předmět: |
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]
Databases Factual Analytical chemistry Information Storage and Retrieval Polyethylene glycol Complex Mixtures Mass spectrometry 01 natural sciences Polyethylene Glycols Matrix (chemical analysis) Automation chemistry.chemical_compound Structural Biology Bifunctional Spectroscopy chemistry.chemical_classification Chromatography 010405 organic chemistry 010401 analytical chemistry Robotics [CHIM.MATE]Chemical Sciences/Material chemistry Polymer PEG 0104 chemical sciences MALDI mass spectrometry Matrix-assisted laser desorption/ionization Solubility chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Monoisotopic mass Algorithms Macromolecule |
| Zdroj: | Journal of The American Society for Mass Spectrometry Journal of The American Society for Mass Spectrometry, Springer Verlag (Germany), 2005, 16, pp.670-678. ⟨10.1016/j.jasmas.2005.01.020⟩ |
| ISSN: | 1044-0305 |
| DOI: | 10.1016/j.jasms.2005.01.020 |
| Popis: | Macromolecules of tunable solubility, used to mimic inert insoluble materials while maintaining solution conditions, allowed the performance of efficient supported organic chemistry and facilitated in situ reaction monitoring. To satisfy the high throughput requirements of automated synthetic processes, organic syntheses carried out on bifunctional polyethylene glycol polymers (PEG(3400)-OH) were monitored step-by-step by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). A protocol was designed to control the ionization mechanism of such polymers exhibiting high affinity for alkali metal cations. Automated, rapid, and reliable data interpretation was performed by an in-house developed visual basic application relying on the sodiated ion accurate monoisotopic mass measurement. The methodology was illustrated through the monitoring of a six-step synthetic scheme. |
| Databáze: | OpenAIRE |
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