Effects of lighting environment on the degeneration of retinal ganglion cells in glutamate/aspartate transporter deficient mice, a mouse model of normal tension glaucoma
| Autor: | Tsutomu Ohashi, Kazuhiko Namekata, Xiaoli Guo, Atsuko Kimura, Chikako Harada, Takayuki Harada |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
GLAST
glutamate/aspartate transporter GLAST deficient mice genetic structures QH301-705.5 GCL ganglion cell layer Lighting environment Biophysics ONL outer nuclear layer Glaucoma QD415-436 IRL inner retinal layer IOP intraocular pressure Biochemistry eye diseases mfERG multifocal electroretinogram RGC retinal ganglion cell sense organs Retinal ganglion cell Biology (General) Neurodegeneration Research Article |
| Zdroj: | Biochemistry and Biophysics Reports Biochemistry and Biophysics Reports, Vol 29, Iss, Pp 101197-(2022) |
| ISSN: | 2405-5808 |
| DOI: | 10.1016/j.bbrep.2021.101197 |
| Popis: | Lighting conditions may affect the development of retinal degenerative diseases such as macular degeneration. In this study, to determine whether the lighting environment affects the progression of degeneration of retinal ganglion cells (RGCs), we examined glutamate/aspartate transporter (GLAST) heterozygous (GLAST+/-) mice, a mouse model of normal tension glaucoma. GLAST+/- mice were reared under a 12-h light-dark cycle (Light/Dark) or complete darkness (Dark/Dark) condition after birth. The total RGC number in the Dark/Dark group was significantly decreased compared with the Light/Dark group at 3 weeks old, while the number of osteopontin-positive αRGCs were similar in both groups. At 6 and 12 weeks old, the total RGC number were not significantly different in both conditions. In addition, the retinal function examined by multifocal electroretinogram were similar at 12 weeks old. These results suggest that lighting conditions may regulate the progression of RGC degeneration in some types of glaucoma. Highlights • Glutamate/aspartate transporter (GLAST) deficient mouse is a model of glaucoma. • Dark conditions accelerated the onset of glaucoma in GLAST deficient mice. • Lighting environment may affect the development of human glaucoma. |
| Databáze: | OpenAIRE |
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