The postnatal age of rat lung fibroblasts influences G1/S phase transition in vitro
| Autor: | Margaret C. Bruce, Walid Al-Jumaily |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Cyclin E Biology Flow cytometry S Phase Rats Sprague-Dawley In vivo Internal medicine medicine Animals Hydroxyurea Fibroblast Lung Cellular Senescence medicine.diagnostic_test Cell growth G1 Phase Cell Biology General Medicine DNA Flow Cytometry Rats Postnatal age Endocrinology medicine.anatomical_structure Blood Animals Newborn Cell culture Stem cell Cell Division Developmental Biology |
| Zdroj: | In vitro cellulardevelopmental biology. Animal. 35(7) |
| ISSN: | 1071-2690 |
| Popis: | In the neonatal rat lung, alveolar development occurs from postnatal Days 4–13, during which time there is a fourfold increase in interstitial fibroblasts. Factors influencing emergence of new septa and cell proliferation associated with septal elongation have yet to be identified, in part because of difficulties inherent in studying this process in vivo. Using flow cytometric analysis of the DNA content of freshly isolated lung fibroblasts, we found that proliferation, as indicated by the percentage of cells in S plus G2/M phases, peaked on postnatal Day 4 (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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