The promoter of DNA puff gene II/9-1 of Sciara coprophila is inducible by ecdysone in late prepupal salivary glands of Drosophila melanogaster
| Autor: | Heidi S. Smith, B Bienz-Tadmor, Susan A. Gerbi |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Ecdysone
Gene Expression Sciara In Vitro Techniques Chromosomes Salivary Glands Animals Genetically Modified chemistry.chemical_compound Gene expression medicine Animals Tissue Distribution Promoter Regions Genetic Gene Reporter gene biology Salivary gland Diptera Age Factors Gene Amplification Pupa Chromosome Mapping General Medicine biology.organism_classification Molecular biology Drosophila melanogaster medicine.anatomical_structure Genes chemistry Larva Ecdysone receptor Research Article |
| Zdroj: | Cell Regulation. 2:875-888 |
| ISSN: | 1044-2030 |
| DOI: | 10.1091/mbc.2.11.875 |
| Popis: | DNA puffs occur in Sciarid salivary gland chromosomes; they are sites of DNA amplification and intense transcription and they appear to encode secreted structural proteins needed for pupation. In this report we have used P-element transformation of Drosophila to study regulation of a Sciara DNA puff gene. We found that a 718-bp promoter fragment of DNA puff gene II/9-1 from Sciara coprophila directs expression of the bacterial reporter gene CAT in late prepupal salivary glands of transgenic Drosophila melanogaster. The identical tissue and analogous stage specificity indicate that some aspects of the ecdysone response are evolutionarily conserved between Drosophila and Sciara. When transgenic salivary glands are cultured in vitro, CAT activity is rapidly induced by ecdysone, suggesting direct control of gene expression by the ecdysone receptor. Putative stage-specific factors limit expression of the chimeric Sciara-CAT gene in transgenic Drosophila to late prepupae but not to third instar larvae when ecdysone titers are also high. |
| Databáze: | OpenAIRE |
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