Evaluation of extraction solutions for biochemical analyses of the proteins in rice grains
| Autor: | Yukari Kagiya, Mayumi Ohnishi-Kameyama, Kazumi Kitta, Gang-hua Lang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Glutens
Protein subunit Liquid-Liquid Extraction Buffers Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Glutelin Protein purification Alpha-Globulins medicine Molecular Biology Polyacrylamide gel electrophoresis Chromatography biology Organic Chemistry Extraction (chemistry) Sodium Dodecyl Sulfate Rice grain Oryza General Medicine Allergens Hydrogen-Ion Concentration Trypsin Molecular Weight Solutions Electrophoresis biology.protein Electrophoresis Polyacrylamide Gel alpha-Amylases Edible Grain Trypsin Inhibitors Biotechnology medicine.drug |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 77(1) |
| ISSN: | 1347-6947 |
| Popis: | The influence of different extraction solutions on the proteins extracted from rice grains was investigated. The largest amounts of salt-soluble proteins were extracted with solutions supplemented with Tris-HCl at pH 8.0. Rice allergens were analyzed by multiplex immunodetection. Except for α-globulin extracted with the solutions at pH 8.0, which showed a low-molecular-weight band besides the main band, no significant solution-dependent difference among the allergens was found. Total proteins were extracted with four kinds of solution. The extraction of the basic subunit of glutelin was found to be SDS-dependent, and more protein was obtained with extraction solutions supplemented with SDS. The contents of α-globulin and α-amylase/trypsin inhibitors were higher in the extracts without SDS than with SDS. We conclude from the present data that, in order to obtain comparable data from rice grain salt-soluble and total protein analyses, differences in the protein extraction efficiency of solutions used should be taken into consideration. |
| Databáze: | OpenAIRE |
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