cBSA-147 for the preparation of bacterial biofilms in a microchannel reactor
| Autor: | Klaus Eisele, Jeck Fei Ng, Tanja Weil, Stephan Jaenicke, Jan Dorn |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Chemistry(all)
General Physics and Astronomy Nanotechnology Physics and Astronomy(all) General Biochemistry Genetics and Molecular Biology Catalysis Biomaterials chemistry.chemical_compound Bioreactors Materials Science(all) Mass transfer Escherichia coli General Materials Science Bovine serum albumin biology Biochemistry Genetics and Molecular Biology(all) Chemistry Cationic polymerization Biofilm Serum Albumin Bovine General Chemistry Cells Immobilized Microfluidic Analytical Techniques Culture Media Chemical engineering Biocatalysis Ethyl acetoacetate Biofilms biology.protein Microreactor |
| Zdroj: | Biointerphases |
| Popis: | Whole cells are attractive biocatalysts, particularly if the reaction requires cofactors or involves multiple transformations. Immobilization of the catalyst is often a prerequisite for continuous processes. The highly cationic chemically modified plasma protein bovine serum albumin (cBSA-147) has been applied for the electrostatically mediated immobilization of the planktonic bacterium E. coli BL21 star (DE3), and the resulting biofilms were superior to those formed on poly-L-lysine coated surfaces. The biocatalyst was immobilized in a capillary column (inside diameter of 530 μm and L=30 m) and evaluated in the enantioselective reduction of ethyl acetoacetate to R-(−)ethyl hydroxybutyrate. In continuous operation in the microreactor format, the productivity of the cells was about 30% higher than that determined in a bench-scale fermentation system. This increase is attributed to the improved mass transfer over short geometrical dimensions. The similarity in the results indicates that studies on a biofilm-coated microreactor can be used for the accelerated collection of data for process optimization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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