Inoculation of Swine with Foot-and-Mouth Disease SAP-Mutant Virus Induces Early Protection against Disease
| Autor: | Marvin J. Grubman, Marcelo Weiss, Camila C. A. Dias, Fayna Diaz-San Segundo, Teresa de los Santos, Eva Pérez-Martín |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Swine
viruses T-Lymphocytes Immunology Enzyme-Linked Immunosorbent Assay Viremia Antibodies Viral Real-Time Polymerase Chain Reaction Microbiology Virus Cell Line Proinflammatory cytokine Immune system Cricetinae Virology medicine Animals Viral shedding Neutralizing antibody DNA Primers Swine Diseases Innate immune system Base Sequence biology Reverse Transcriptase Polymerase Chain Reaction Viral Vaccines biology.organism_classification medicine.disease Foot-and-Mouth Disease Virus Foot-and-Mouth Disease Insect Science Mutation biology.protein Pathogenesis and Immunity Foot-and-mouth disease virus |
| Zdroj: | Journal of Virology. 86:1316-1327 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Foot-and-mouth disease virus (FMDV) leader proteinase (L pro ) cleaves itself from the viral polyprotein and cleaves the translation initiation factor eIF4G. As a result, host cell translation is inhibited, affecting the host innate immune response. We have demonstrated that L pro is also associated with degradation of nuclear factor κB (NF-κB), a process that requires L pro nuclear localization. Additionally, we reported that disruption of a conserved protein domain within the L pro coding sequence, SAP mutation, prevented L pro nuclear retention and degradation of NF-κB, resulting in in vitro attenuation. Here we report that inoculation of swine with this SAP-mutant virus does not cause clinical signs of disease, viremia, or virus shedding even when inoculated at doses 100-fold higher than those required to cause disease with wild-type (WT) virus. Remarkably, SAP-mutant virus-inoculated animals developed a strong neutralizing antibody response and were completely protected against challenge with WT FMDV as early as 2 days postinoculation and for at least 21 days postinoculation. Early protection correlated with a distinct pattern in the serum levels of proinflammatory cytokines in comparison to the levels detected in animals inoculated with WT FMDV that developed disease. In addition, animals inoculated with the FMDV SAP mutant displayed a memory T cell response that resembled infection with WT virus. Our results suggest that L pro plays a pivotal role in modulating several pathways of the immune response. Furthermore, manipulation of the L pro coding region may serve as a viable strategy to derive live attenuated strains with potential for development as effective vaccines against foot-and-mouth disease. |
| Databáze: | OpenAIRE |
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