In vitro control release, cytotoxicity assessment and cellular uptake of methotrexate loaded liquid-crystalline folate nanocarrier
| Autor: | Vivekanandan Perumal, Sanat Mohanty, Mohita Upadhyay, Rahul Misra |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Light
Pharmacology Fluorescence HeLa Mice Folic Acid Hypromellose Derivatives In vivo Cations Animals Humans Scattering Radiation MTT assay Particle Size Cytotoxicity Drug Carriers Cell Death biology Chemistry Folate Receptors GPI-Anchored General Medicine biology.organism_classification Endocytosis In vitro Liquid Crystals Cross-Linking Reagents Methotrexate Folate receptor Delayed-Action Preparations Cancer cell NIH 3T3 Cells Nanoparticles Nanocarriers HeLa Cells |
| Zdroj: | Biomedicine & Pharmacotherapy. 69:102-110 |
| ISSN: | 0753-3322 |
| Popis: | Folate molecules self-assemble in the form of stacks to form liquid-crystalline solutions. Nanocarriers from self-assembled folates are composed of highly ordered structures, which offer high encapsulation of drug (95-98%), controlled drug release rates, active cellular uptake and biocompatibility. Recently, we have shown that the release rates of methotrexate can be controlled by varying the size of nanoparticles, cross-linking cation and cross-linking concentration. The present study reports the in vitro cytotoxic behavior of methotrexate loaded liquid-crystalline folate nanoparticles on cultured HeLa cells. Changing drug release rates can influence cytotoxicity of cancer cells. Therefore, to study the correlation of release rate and cytotoxic behavior, the effect of release controlling parameters on HeLa cells was studied through MTT assay. It is reported that by controlling the methotrexate release, the survival rates of HeLa cells can be controlled. Released methotrexate kills HeLa cells as effectively as free methotrexate solution. The co-culture based in vitro cellular uptake study through fluorescence microscopy on folate receptor positive and negative cancer cells shows that the present nanocarrier has the potential to distinguish cancer cells from normal cells. Overall, the present study reports the in vitro performance of self-assembled liquid-crystalline folate nanoparticles, which will be a platform for further in vivo studies and clinical trials. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect