Comparison of BD GeneOhm Cdiff and Seegene Seeplex ACE PCR Assays Using Toxigenic Clostridium difficile Culture for Direct Detection of tcdB from Stool Specimens
| Autor: | Bo-Moon Shin, Eun Young Kuak, Se Jin Mun, Soo Jin Yoo |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Bacteriological Techniques Microbial toxins Clostridioides difficile Bacterial Toxins Pcr assay Bacteriology Clostridium Infections Biology Clostridium difficile culture Polymerase Chain Reaction Sensitivity and Specificity Predictive value Microbiology Bacterial protein Feces Bacterial Proteins Molecular Diagnostic Techniques Predictive Value of Tests Humans Molecular diagnostic techniques |
| Zdroj: | Journal of Clinical Microbiology. 50:3765-3767 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.01440-12 |
| Popis: | We evaluated the performances of 2 PCR assays (BD GeneOhm and Seegene ACE) for direct detection of tcdB from stool specimens. The concordance rate between BD and Seegene was 96.3%. The sensitivities, specificities, positive predictive values (PPVs), and negative predictive values (NPVs) of BD and Seegene were 95.7%, 96.5%, 91.8%, and 98.2% and 90.0%, 97.1%, 92.6%, and 96.0%, respectively. |
| Databáze: | OpenAIRE |
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