In Vivo Imaging of Activated Estrogen Receptors in Utero by Estrogens and Bisphenol A
| Autor: | Paul T. van der Saag, Bart van der Burg, Roel J. Arends, J.G. Lemmen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
endocrine system Health Toxicology and Mutagenesis bisphenol A Diethylstilbestrol Estrogen receptor Mice Transgenic Biology in utero Transactivation Mice Phenols In vivo Genes Reporter Pregnancy Internal medicine medicine Animals Estrogens Non-Steroidal Benzhydryl Compounds Receptor reporter mice Maternal-Fetal Exchange Research Public Health Environmental and Occupational Health In vitro toxicology Gene Expression Regulation Developmental Reproducibility of Results Estrogens Articles Environmental Estrogen in vivo Endocrinology Receptors Estrogen In utero Environmental Pollutants Female hormones hormone substitutes and hormone antagonists medicine.drug estrogen receptor |
| Zdroj: | Environmental Health Perspectives |
| ISSN: | 1552-9924 0091-6765 |
| Popis: | Environmental estrogens are of particular concern when exposure occurs during embryonic development. Although there are good models to study estrogenic activity of chemicals in adult animals, developmental exposure is much more difficult to test. The weak estrogenic activity of the environmental estrogen bisphenol A (BPA) in embryos is controversial. We have recently generated transgenic mice that carry a reporter construct with estrogen-responsive elements coupled to luciferase. We show that, using this in vivo model in combination with the IVIS imaging system, activation of estrogen receptors (ERs) by maternally applied BPA and other estrogens can be detected in living embryos in utero. Eight hours after exposure to 1 mg/kg BPA, ER transactivation could be significantly induced in the embryos. This was more potent than would be estimated from in vitro assays, although its intrinsic activity is still lower than that of diethylstilbestrol and 17beta-estradiol dipropionate. On the basis of these results, we conclude that the estrogenic potency of BPA estimated using in vitro assays might underestimate its estrogenic potential in embryos. |
| Databáze: | OpenAIRE |
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