Comparison of human gut microbiota in control subjects and patients with colorectal carcinoma in adenoma: Terminal restriction fragment length polymorphism and next-generation sequencing analyses
| Autor: | Hidekazu Inoue, Junichiro Tanaka, Kojiro Takase, Kazushi Sugimoto, Isao Moritani, Yoshiyuki Takei, Chika Kasai, Katsuya Shiraki, Masahiko Tameda, Yumi Oya, Masaaki Ito |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Adenoma Adult Male Cancer Research medicine.medical_specialty Colorectal cancer Colon Adenoma Biology Gut flora Gastroenterology DNA Ribosomal 03 medical and health sciences Feces Japan Internal medicine RNA Ribosomal 16S medicine Carcinoma Humans Eubacterium Bacteria High-Throughput Nucleotide Sequencing General Medicine Sequence Analysis DNA Middle Aged biology.organism_classification medicine.disease digestive system diseases Healthy Volunteers Gastrointestinal Microbiome stomatognathic diseases 030104 developmental biology Oncology Fusobacterium Immunology Female Colorectal Neoplasms Actinomyces Polymorphism Restriction Fragment Length |
| Zdroj: | Oncology reports. 35(1) |
| ISSN: | 1791-2431 |
| Popis: | Colorectal cancer (CRC) is the third leading cause of cancer-related deaths in Japan. The etiology of CRC has been linked to numerous factors including genetic mutation, diet, life style, inflammation, and recently, the gut microbiota. However, CRC-associated gut microbiota is still largely unexamined. This study used terminal restriction fragment length polymorphism (T-RFLP) and next-generation sequencing (NGS) to analyze and compare gut microbiota of Japanese control subjects and Japanese patients with carcinoma in adenoma. Stool samples were collected from 49 control subjects, 50 patients with colon adenoma, and 9 patients with colorectal cancer (3/9 with invasive cancer and 6/9 with carcinoma in adenoma) immediately before colonoscopy; DNA was extracted from each stool sample. Based on T-RFLP analysis, 12 subjects (six control and six carcinoma in adenoma subjects) were selected; their samples were used for NGS and species-level analysis. T-RFLP analysis showed no significant differences in bacterial population between control, adenoma and cancer groups. However, NGS revealed that i), control and carcinoma in adenoma subjects had different gut microbiota compositions, ii), one bacterial genus (Slackia) was significantly associated with the control group and four bacterial genera (Actinomyces, Atopobium, Fusobacterium, and Haemophilus) were significantly associated with the carcinoma-in-adenoma group, and iii), several bacterial species were significantly associated with each type (control: Eubacterium coprostanoligens; carcinoma in adenoma: Actinomyces odontolyticus, Bacteroides fragiles, Clostridium nexile, Fusobacterium varium, Haemophilus parainfluenzae, Prevotella stercorea, Streptococcus gordonii, and Veillonella dispar). Gut microbial properties differ between control subjects and carcinoma-in-adenoma patients in this Japanese population, suggesting that gut microbiota is related to CRC prevention and development. |
| Databáze: | OpenAIRE |
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