Evaluation of random amplified polymorphic DNA and multiple-locus variable number tandem repeat analyses for Mycoplasma cynos
| Autor: | Aslı Sakmanoğlu, Ali Uslu, Yasemin Pinarkara, Zafer Sayin, Osman Erganiş, Uçkun Sait Uçan |
|---|---|
| Přispěvatelé: | Selçuk Üniversitesi, Veteriner Fakültesi, Temel Bilimler Bölümü, Sakmanoglu, Aslı., Sayin, Zafer., Pinarkara, Yasemin., Uslu, Ali., Ucan, Uckun Sait., Erganis, Osman. |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
DNA Bacterial Genotype Genotyping Techniques Minisatellite Repeats Multiple Loci VNTR Analysis Biology medicine.disease_cause Microbiology Polymerase Chain Reaction 03 medical and health sciences Dogs Mycoplasma Tandem repeat RAPD medicine Mycoplasma cynos Animals Mycoplasma Infections Molecular Biology Genotyping 030304 developmental biology DNA Primers Genetics 0303 health sciences Base Composition 030306 microbiology Bronchoalveolar lavage fluid MLVA food and beverages Genetic Variation TP-RAPD bacterial infections and mycoses Subtyping Random Amplified Polymorphic DNA Technique Variable number tandem repeat Tandem Repeat Sequences GC-content |
| Popis: | WOS: 000470944100001 PubMed: 30981711 Mycoplasma spp. can cause diseases of the respiratory system as well as urogenital infections, infertility, and anemia. The members of this genus have a low G + C content compared to other bacteria. Because primers used in the random amplified polymorphic DNA (RAPD) technique are only 10 bp long and have high GC content, this method can be inadequate for genotyping Mycoplasma spp. isolates. The aim of this study was to develop and evaluate multiple-locus variable number tandem repeat analysis (MLVA) and two-primer RAPD (TP-RAPD) procedures for subtyping Mycoplasma cynos isolates. A total of 55 M. cynos isolates obtained from 162 bronchoalveolar lavage fluid samples from shelter and pet dogs were used in this study. Seventy-four tandem repeat regions were detected in the M. cynos genome, and two of these loci were determined to be suitable and used for development of the MLVA scheme. The results of variable number tandem repeat (VNTR) analysis and TP-RAPD-PCR were compared with RAPD-PCR. The discriminatory power of TP-RAPD-PCR (Hunter-Gaston diversity index [HGDI] = 0.84) was higher than those of RAPD-PCR (HGDI = 0.727), VNTR1 (HGDI = 0.8), and VNTR3 (HGDI = 0.757). We observed that the TPRAPD-PCR and MLVA methods provide clearer data and are more successful in determining genetic diversity, in contrast to the RAPD-PCR method for this species. Coordinatorship for Scientific Research Projects of Selcuk University [SUBAPK 17401041] This study was part of a research project supported by the Coordinatorship for Scientific Research Projects of Selcuk University (SUBAPK 17401041, Konya, Turkey). This study previously presented partly as an oral presentation at the XIII. Veterinary Microbiology Congress (October 2018-Antalya/Turkey). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|