Increased Malbranchea pulchella β-glucosidase production and its application in agroindustrial residue hydrolysis: A research based on experimental designs
Autor: | Paulo Ricardo Heinen, Carem Gledes Vargas Rechia, Lummy Maria Oliveira Monteiro, Josana Maria Messias, Vanessa Elisa Pinheiro, Maria de Lourdes Teixeira de Moraes Polizeli, Marcos Silveira Buckeridge, Ana Claudia Vici |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
Limiting factor 0303 health sciences Residue (complex analysis) Coefficient of determination Thermophilic fungus Biomass food and beverages Mixture design Pulp and paper industry Composite central design 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Hydrolysis chemistry 010608 biotechnology Ethanol fuel Cellulose Bagasse Second-generation ethanol TP248.13-248.65 030304 developmental biology Biotechnology |
Zdroj: | Biotechnology Reports, Vol 30, Iss, Pp e00618-(2021) |
Popis: | β-Glucosidases are a limiting factor in the conversion of cellulose to glucose for the subsequent ethanol production. Here, β-glucosidase production by Malbranchea pulchella was optimized using Composite Central Designs and Response Surface Methodologies from a medium designed. The coefficient of determination (R2) was 0.9960, F-value was very high, and the lack of fit was found to be non-significant. This indicates a statistic valid and predictive result. M. pulchella enzymatic extract was successfully tested as an enzymatic cocktail in a mixture design using sugarcane bagasse, soybean hull and barley bagasse. We proved that the optimization of the β-glucosidase production and the application in hydrolysis using unexpansive biomass and agricultural wastes can be accomplished by means of statistical methodologies. The strategy presented here can be useful for the improvement of enzyme production and the hydrolysis process, arising as an alternative for bioeconomy. |
Databáze: | OpenAIRE |
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