Additional file 2 of Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution

Autor: Matsuo, Yoshiyuki, Komiya, Shinnosuke, Yasumizu, Yoshiaki, Yasuoka, Yuki, Mizushima, Katsura, Takagi, Tomohisa, Kryukov, Kirill, Aisaku Fukuda, Morimoto, Yoshiharu, Naito, Yuji, Okada, Hidetaka, Bono, Hidemasa, Nakagawa, So, Hirota, Kiichi
Rok vydání: 2021
Předmět:
DOI: 10.6084/m9.figshare.13647002
Popis: Additional file 2 Fig. S1. Sequence heterogeneities of the 27F primer-annealing site in 16S rRNA genes. Fig. S2. Evaluation of 16S rRNA PCR primers for identification of bacterial species. Fig. S3. Classification results of a mock community analyzed by the different bioinformatics workflows. Fig. S4. Effect of read number on taxonomic classification. Fig. S5. Species composition of a mock community analyzed by FASTQ 16S workflow. Fig. S6. Rarefaction curves of observed OTUs in V3-V4 16S rRNA gene amplicon sequencing of human fecal samples using the MiSeq™ platform. Fig. S7. Species composition of Blautia in human fecal samples. Fig. S8. Species composition of Bacteroides in human fecal samples. Fig. S9. Deviations in the relative abundances of Bifidobacterium species in human fecal samples. Fig. S10. Comparison of species composition of fecal Bifidobacterium between classification methods.
Databáze: OpenAIRE