COMPREHENSIVE ANALYSIS OF CHEMOKINES AND CYTOKINES SECRETED IN THE PERITONEAL CAVITY DURING LAPAROTOMY

Autor:	Taeko Dohi, Yuki I. Kawamura, Toshihiko Okada, Rei Kawashima, Yutaka J. Kawamura, Noriko Mizutani, Fumio Konishi, Tomoyuki Oshio
Rok vydání:	2012
Předmět:	Adult Male Chemokine Pathology medicine.medical_specialty medicine.medical_treatment Clinical Biochemistry Immunology Peripheral blood mononuclear cell CCL5 Peritoneal cavity cytokine medicine Ascitic Fluid Humans Immunology and Allergy Peritoneal Lavage Chemokine CCL5 Cells Cultured Aged multiplex beads assay Laparotomy biology Chemotaxis Peritoneal fluid chemokine Middle Aged Recombinant Proteins Cell aggregation Medical Laboratory Technology peritoneal fluid medicine.anatomical_structure Cytokine Leukocytes Mononuclear biology.protein Female Chemokines Research Article
Zdroj:	Journal of Immunoassay & Immunochemistry
ISSN:	1532-4230 1532-1819
DOI:	10.1080/15321819.2011.638409
Popis:	We recently found that chemokine-driven peritoneal cell aggregation is the primary mechanism of postoperative adhesion in a mouse model. To investigate this in humans, paired samples of peritoneal lavage fluid were obtained from seven patients immediately after incision (preoperative) and before closure (postoperative), and were assayed for the presence of 27 cytokines and chemokines using multiplex beads assay. As a result, IL-6 and CCL5 showed the most striking increase during operation. Recombinant CCL5 or lavage fluid induced chemotaxis of human peripheral blood mononuclear cells. We propose that CCL5 is possibly involved in the mechanism of postoperative adhesion in humans.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95d83b643b5cff971747e0175a1ecc89 https://doi.org/10.1080/15321819.2011.638409 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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