The glutamate uptake regulatory protein (Grp) of Zymomonas mobilis and its relation to the global regulator Lrp of Escherichia coli

Autor: B Tolner, Reinhard Krämer, Berend Poolman, N. Peekhaus
Rok vydání: 1995
Předmět:
Molecular Sequence Data
Restriction Mapping
Gene Dosage
Glutamic Acid
medicine.disease_cause
Regulon
Microbiology
Zymomonas mobilis
Bacterial Proteins
Genes
Regulator
Operon
Escherichia coli
Leucine-responsive regulatory protein
medicine
Amino Acid Sequence
Cloning
Molecular
Molecular Biology
Regulation of gene expression
Aspartic Acid
Zymomonas
Base Sequence
Sequence Homology
Amino Acid
Symporters
biology
Escherichia coli Proteins
Genetic Complementation Test
Glutamate receptor
Biological Transport
Gene Expression Regulation
Bacterial
Glutamic acid
biology.organism_classification
Leucine-Responsive Regulatory Protein
DNA-Binding Proteins
Biochemistry
Genes
Bacterial
Regulatory sequence
biology.protein
Carrier Proteins
hormones
hormone substitutes
and hormone antagonists
Transcription Factors
Research Article
Zdroj: Journal of Bacteriology. 177:5140-5147
ISSN: 1098-5530
0021-9193
DOI: 10.1128/jb.177.17.5140-5147.1995
Popis: After being expressed in Escherichia coli JC5412, which is defective in glutamate transport, a Zymomonas mobilis gene which enabled this strain to grow on glutamate was cloned. This gene encodes a protein with 33% amino acid identity to the leucine-responsive regulatory protein (Lrp) of E. coli. Although overall glutamate uptake in E. coli was increased, the protein encoded by the cloned fragment repressed the secondary H+/glutamate transport system GltP by interaction with the promoter region of the gltP gene. It also repressed the secondary, H(+)-coupled glutamate uptake system of Z. mobilis, indicating that at least one role of this protein in Z. mobilis is to regulate glutamate transport. Consequently, it was designated Grp (for glutamate uptake regulatory protein). When expressed in E. coli, Grp repressed the secondary H+/glutamate transport system GltP by binding to the regulatory regions of the gltP gene. An lrp mutation in E. coli was complemented in trans with respect to the positive expression regulation of ilvIH (coding for acetohydroxy acid synthase III) by a plasmid which carries the grp gene. The expression of grp is autoregulated, and in Z. mobilis, it depends on growth conditions. The putative presence of a homolog of Grp in E. coli is discussed.
Databáze: OpenAIRE
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