Follicular Helper T Cell Derived Exosomes Promote B Cell Proliferation and Differentiation in Antibody-Mediated Rejection after Renal Transplantation

Autor:	Lili Bi, Li Xiao, Zhen Wang, Jintao Yang, Bingyi Shi, Yeyong Qian, Xiuyun He
Rok vydání:	2019
Předmět:	Adult Graft Rejection Male medicine.medical_specialty Article Subject T cell Plasma Cells lcsh:Medicine Human leukocyte antigen Biology Exosomes CXCR3 General Biochemistry Genetics and Molecular Biology CXCR5 Organ transplantation Isoantibodies Follicular phase medicine Humans Cell Proliferation HLA-G Antigens General Immunology and Microbiology lcsh:R T-Lymphocytes Helper-Inducer General Medicine Middle Aged Kidney Transplantation Microvesicles Transplantation medicine.anatomical_structure Gene Expression Regulation Cancer research Female Research Article
Zdroj:	BioMed Research International BioMed Research International, Vol 2019 (2019)
ISSN:	2314-6141 2314-6133
Popis:	Follicular helper T cells (Tfh cells) are closely related to the occurrence and development of antibody-mediated rejection (AMR) after renal transplantation. Exosomes play a key role in the rejection after organ transplantation. However, whether Tfh-derived exosomes are involved in AMR has not been reported. We collected peripheral blood from 42 kidney transplant patients and found no significant differences in CD4+CXCR5+ and CD4+CXCR5+CXCR3+CCR6-exosomes between AMR and non-AMR groups, whereas the proportion of CD4+CXCR5+CXCR3-exosomes was significantly higher in AMR group than that in non-AMR group; CTLA-4 expression of CD4+CXCR5+exosomes was significantly lower in AMR group than that in non-AMR group. HLA-G expression was not significantly different between two groups. We further separated CD4+CXCR5+cells from patients by magnetic beads. Coculture experiments showed that Tfh cell-derived exosomes in AMR patients significantly promoted B cell proliferation and differentiation, compared with non-AMR group, the percentage of B cells and plasma cells increased by 87.52% and 110.2%, respectively. In conclusion, our study found that Tfh cell-derived exosomes could promote the proliferation and differentiation of B cells and they may play an important role in the development of AMR after renal transplantation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95d611945aadbf27b5bb49b18501a2b2 https://doi.org/10.1155/2019/6387924 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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