Critical evaluation of the DNA-methylation markers ABCG1 and SREBF1 for Type 2 diabetes stratification
| Autor: | Hendrik Lehnert, Olena Ohlei, Meike Kasten, Henriette Kirchner, Stefan Wolter, Oliver Mann, Christine Klein, Fabian Kilpert, Georg Brabant, Helen Sievert, Martina Grohs, Cathleen Geißler, Alexander T. El Gammal, Ulrike M. Krämer, Christin Krause |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Genetic Markers
0301 basic medicine Oncology Cancer Research medicine.medical_specialty Adipose tissue Type 2 diabetes 03 medical and health sciences 0302 clinical medicine Diabetes mellitus Internal medicine Genetics medicine Humans Insulin SNP ATP Binding Cassette Transporter Subfamily G Member 1 Genetic association biology Methylation DNA Methylation medicine.disease 030104 developmental biology Diabetes Mellitus Type 2 ABCG1 030220 oncology & carcinogenesis DNA methylation biology.protein Sterol Regulatory Element Binding Protein 1 |
| Zdroj: | Epigenomics. 11:885-897 |
| ISSN: | 1750-192X 1750-1911 0650-0161 |
| DOI: | 10.2217/epi-2018-0159 |
| Popis: | Aim: Validation of epigenome-wide association studies is sparse. Therefore, we evaluated the methylation markers cg06500161 ( ABCG1) and cg11024682 ( SREBF1) as classifiers for diabetes stratification. Patients & methods: DNA methylation was measured in blood (n = 167), liver (n = 99) and visceral adipose tissue (n = 99) of nondiabetic or Type 2 diabetic subjects by bisulfite pyrosequencing. Results: DNA methylation at cg11024682 in blood and liver correlated with BMI. Methylation at cg06500161 was influenced by the adjacent SNP rs9982016. Insulin-resistant and sensitive subjects could be stratified by DNA methylation status in blood or visceral adipose tissue. Conclusion: DNA methylation at both loci in blood presents a promising approach for risk group stratification and could be valuable for personalized Type 2 diabetes risk prediction in the future. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|