Nationwide study of Escherichia coli producing extended-spectrum ß-lactamases TEM, SHV and CTX-M in Turkey
| Autor: | Pervin Ozlem Balci, Ahmet Caliskan, Cemal Sandalli, Zeynep Cizmeci, Mehmet Firat, Ayşegül Saral, Fatma Sari, Metin Sancaktar, Emine Esra Budak, Yasemin Ay Altintop, Ayşegül Çopur Çiçek, Ekrem Yasar, Sibel Ak, Ayşe Ertürk, Azer Özad Düzgün, Nazan Yildiz, Osman Birol Ozgumus |
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| Přispěvatelé: | Copur Cicek, A., Department of Medical Microbiology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey -- Saral, A., Department of Biology, Faculty of Arts and Sciences, Artvin Coruh University, Artvin, Turkey -- Ozad Duzgun, A., Department of Biology, Faculty of Arts and Sciences, Giresun University, Giresun, Turkey -- Yasar, E., Laboratory of Microbiology, Diyarbakir Pediatrics Hospital, Diyarbakir, Turkey -- Cizmeci, Z., Department of Medical Microbiology, Kecioren Training and Research Hospital, Ankara, Turkey -- Ozlem Balci, P., Laboratory of Microbiology, Tokat State Hospital, Tokat, Turkey -- Sari, F., Laboratory of Microbiology, Denizli Servergazi State Hospital, Denizli, Turkey -- Firat, M., Ozel OSM Ortadogu Hospital, Infectious Diseases and Clinical Microbiology, Sanliurfa, Turkey -- Altintop, Y.A., Laboratory of Microbiology, Nigde State Hospital, Nigde, Turkey -- Ak, S., Laboratory of Microbiology, Malatya State Hospital, Malatya, Turkey -- Caliskan, A., Laboratory of Microbiology, Kahramanmaras Necip Fazil State Hospital, Kahramanmaras, Turkey -- Yildiz, N., Laboratory of Microbiology, Konya Beyhekim State Hospital, Konya, Turkey -- Sancaktar, M., Laboratory of Microbiology, Trabzon Hackali Baba State Hospital, Trabzon, Turkey -- Esra Budak, E., Microbiology and Molecular Biology Research Laboratory, Department of Biology, Faculty of Arts and Sciences, Recep Tayyip Erdogan University, Rize 53100, Turkey -- Erturk, A., Department of Medical Microbiology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey -- Birol Ozgumus, O., Department of Medical Microbiology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey -- Sandalli, C., Microbiology and Molecular Biology Research Laboratory, Department of Biology, Faculty of Arts and Sciences, Recep Tayyip Erdogan University, Rize 53100, Turkey, 0-Belirlenecek, RTEÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Çiçek, Ayşegül Çopur, Özgüm, Osman Birol, Sandallı, Cemal, Belirlenecek |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
DNA
Bacterial SHV-11 Turkey Sequence analysis ß-lactamase Urine Biology medicine.disease_cause Polymerase Chain Reaction beta-Lactamases Microbiology law.invention law TEM-1b Drug Discovery medicine Escherichia coli polycyclic compounds Humans Polymerase chain reaction Escherichia coli Infections Pharmacology CTX-M2 Beta-Lactamase β lactamases ComputerSystemsOrganization_COMPUTER-COMMUNICATIONNETWORKS CTX-M1 Sequence Analysis DNA biochemical phenomena metabolism and nutrition bacterial infections and mycoses ComputingMilieux_MANAGEMENTOFCOMPUTINGANDINFORMATIONSYSTEMS ComputingMethodologies_PATTERNRECOGNITION Pleural fluid bacteria Sputum medicine.symptom InformationSystems_MISCELLANEOUS beta-lactamase |
| Popis: | PubMed ID: 23838745 Four hundred and forty extended-spectrum ß-lactamase (ESBL)-producing Escherichia coli isolates were collected from 10 different hospitals in Turkey between 2011 and 2012. Clinical specimens consisted of urine (80.45%), blood (6.59%), cerebrospinal fluid (1.13%), pleural fluid (2.95%), wound (4.31%) and sputum (4.54%). ESBL-coding genes (CTX-M1, CTX-M2, TEM, SHV) were detected by PCR. According to the PCR and sequencing results, CTX-M1 was the most prevalent ß-lactamase 83.18% (366/440), followed by TEM 44.09% (194/440), CTX-M2 31.81% (140/440) and SHV 1.81% (8/440). Sequencing results showed that TEM and SHV types were TEM-1b and SHV-11, respectively. Rate of the strains harboring only CTX-M1, CTX-M2, TEM-1b and SHV-11 were 30.90%, 3.63%, 2.27% and 0.23%, respectively. Rate of the strains harboring the combinations of CTX-M1-CTX-M2, CTX-M1-CTX-M2-TEM-1b, CTX-M2-TEM-1b, CTX-M1-TEM-1b, CTX-M1-CTX-M2-TEM-1b-SHV-11, CTX-M1-TEM-1b-SHV-11, CTX-M1-SHV-11, CTX-M1-CTX-M2-SHV-11, CTX-M2-SHV-11, CTX-M2-TEM-1b-SHV-11, TEM-1b-SHV-11 were 12.95%, 11.59%, 2.95%, 26.13%, 0.45%, 0.68%, 0.22%, 0.22%, 0%, 0% and 0%, respectively. This is a nationwide study of ESBL-producing E. coli in Turkey. These results shows that CTX-M1 group is the most common type of class A ß-lactamases among ESBL-producing E. coli strains in Turkey. © 2013 Japan Antibiotics Research Association. Recep Tayyip Erdogan Üniversitesi Fundamental Research Fund of Shandong University This work was supported by Recep Tayyip Erdogan University Research Fund Grants BAP-2012.106.01.11 and BAP-2011.102.03.3. |
| Databáze: | OpenAIRE |
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