Effect of low-density lipoproteins and trehalose on the quality of cryopreserved bovine semen
| Autor: | Giovanni Restrepo-Betancur, Jorge Gómez-Oquendo, Alexandra Úsuga-Suárez, Elizabeth Varela-Giraldo, Juan E. Duque-Cortés |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
food.ingredient
Cryoprotectant medicine.medical_treatment Semen cryopreservation SF1-1100 Cryopreservation law.invention Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine food law Yolk low-density lipoproteins medicine bull trehalose 030219 obstetrics & reproductive medicine General Veterinary Chemistry cryoprotectant Artificial insemination Extender 0402 animal and dairy science semen 04 agricultural and veterinary sciences 040201 dairy & animal science Trehalose Sperm Animal culture egg yolk extender Animal Science and Zoology |
| Zdroj: | Revista Colombiana de Ciencias Pecuarias, Vol 34, Iss 3, Pp 200-211 (2020) |
| ISSN: | 2256-2958 0120-0690 |
| DOI: | 10.17533/udea.rccp.v34n3a03 |
| Popis: | Background: In artificial insemination, chicken egg yolk is added to bovine semen to protect it during the cryopreservation process, although it contains substances that can affect the microbiological quality and metabolism of sperm. Objective: To evaluate post-thaw quality of bovine cryopreserved semen added with centrifuged and non-centrifuged egg yolk, low-density lipoproteins (LDL), and trehalose (T). Methods: Ten ejaculates from five bulls were cryopreserved under the treatments T1: pure egg yolk (PEY) at 20% v/v, T2: centrifuged egg yolk (CEY) at 20% v/v, T3: LDL at 8% v/v, T4: T at 100 mM, and T5: T at 100 mM plus LDL at 8% v/v (TLDL). Spermatic motility and kinetics, functional membrane integrity (FMI), structural membrane integrity (SMI), sperm vitality (SV) and abnormal morphology (AM) were assessed using the Sperm Class Analyzer (SCA®) system, hypoosmotic test (HOST), SYBR/PI probes, and eosin–nigrosin staining, respectively. A completely randomized design was used. Normal distribution of the variables was validated through the Kolmogórov– Smirnov test. A generalized linear model was used to determine sources of variation. Means were compared using the Tukey test. Results: Inclusion of CEY or LDL had a similar effect on sperm protection, and were superior for motility, kinetics and membrane integrity compared to the other treatments (p0.05). Conclusions: Egg yolk can be replaced by centrifuged egg yolk or low-density lipoproteins in the freezing extender used for bovine semen used in artificial insemination. |
| Databáze: | OpenAIRE |
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