TonB is essential for virulence in avian pathogenic Escherichia coli
| Autor: | Karen M. Holden, Philip F. Markham, Marc S. Marenda, Glenn F. Browning, Amir H. Noormohammadi |
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| Rok vydání: | 2012 |
| Předmět: |
animal structures
Respiratory System Immunology Mutant Mutagenesis (molecular biology technique) Virulence Biology medicine.disease_cause Microbiology chemistry.chemical_compound Plasmid Pathogenic Escherichia coli Gene Order Escherichia coli medicine Animals Immunology and Allergy Escherichia coli Infections Genetics General Veterinary Escherichia coli Proteins Membrane Proteins General Medicine biology.organism_classification Phenotype Infectious Diseases Streptonigrin chemistry Mutation Aerobactin Chickens |
| Zdroj: | Comparative Immunology, Microbiology and Infectious Diseases. 35:129-138 |
| ISSN: | 0147-9571 |
| DOI: | 10.1016/j.cimid.2011.12.004 |
| Popis: | Avian pathogenic Escherichia coli (APEC) strains have multiple iron-uptake systems that facilitate adaptation to iron-restricted environments and are believed to assist in colonisation of the host. These systems include several TonB-dependent transporters of ferri-siderophores encoded by the chromosome and the large virulence plasmid common to APECs. The tonB gene of the virulent APEC strain E956 was replaced with a selectable antibiotic resistance marker using Lambda Red recombinase mutagenesis. The phenotype of the ΔtonB E956 mutant was compared to the parent strain under various culture conditions and in chickens experimentally infected via the respiratory route. The mutant was resistant to streptonigrin, impaired in its ability to adapt to growth in iron-depleted medium and had greater tolerance of oxidative stress than the parental strain. The mutant was avirulent in chickens, did not affect the growth of chicks and colonisation was mostly limited to the trachea. This study has demonstrated that TonB is essential for virulence in APEC. |
| Databáze: | OpenAIRE |
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