PINK1-Interacting Proteins: Proteomic Analysis of Overexpressed PINK1
| Autor: | Aleksandar Rakovic, Slobodanka Orolicki, Anne Grünewald, Sarah Hofmann, Christine Klein, Lisa Voges, Katja Lohmann |
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| Rok vydání: | 2010 |
| Předmět: |
Genetics
Tandem affinity purification Candidate gene medicine.diagnostic_test Article Subject Neuroscience (miscellaneous) PINK1 Biology Parkin lcsh:RC346-429 Cell biology Blot Psychiatry and Mental health Western blot Mitophagy medicine HSP60 Neurology (clinical) lcsh:Neurology. Diseases of the nervous system Research Article |
| Zdroj: | Parkinson's Disease Parkinson's Disease, Vol 2011 (2011) BASE-Bielefeld Academic Search Engine |
| ISSN: | 2042-0080 |
| Popis: | Recent publications suggest that the Parkinson's disease- (PD-) related PINK1/Parkin pathway promotes elimination of dysfunctional mitochondria by autophagy. We used tandem affinity purification (TAP), SDS-PAGE, and mass spectrometry as a first step towards identification of possible substrates for PINK1. The cellular abundance of selected identified interactors was investigated by Western blotting. Furthermore, one candidate gene was sequenced in 46 patients with atypical PD. In addition to two known binding partners (HSP90, CDC37), 12 proteins were identified using the TAP assay; four of which are mitochondrially localized (GRP75, HSP60, LRPPRC, and TUFM). Western blot analysis showed no differences in cellular abundance of these proteins comparingPINK1mutant and control fibroblasts. When sequencingLRPPRC, four exonic synonymous changes and 20 polymorphisms in noncoding regions were detected. Our study provides a list of putative PINK1 binding partners, confirming previously described interactions, but also introducing novel mitochondrial proteins as potential components of the PINK1/Parkin mitophagy pathway. |
| Databáze: | OpenAIRE |
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