Lack of association between intact/deletion polymorphisms of the APOBEC3B gene and HIV-1 risk

Autor: Hirotaka Ode, Akifumi Takaori-Kondo, Noriyo Kaneko, Yoshiyuki Yokomaku, Seiichi Ichikawa, Junji Imamura, Mayumi Imahashi, Dai Watanabe, Makoto Utsumi, Kei Sato, Takuma Shirasaka, Wataru Sugiura, Yasumasa Iwatani, Taisuke Izumi, Kazuhiro Matsuoka, Tomoki Naoe, Takashi Masaoka, Yoshio Koyanagi
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Male
Viral Diseases
lcsh:Medicine
HIV Infections
Pathogenesis
Pathology and Laboratory Medicine
Cohort Studies
Immunodeficiency Viruses
Japan
Polymorphism (computer science)
Genotype
Medicine and Health Sciences
lcsh:Science
education.field_of_study
Multidisciplinary
virus diseases
Viral Load
Middle Aged
Infectious Diseases
Medical Microbiology
Viral Pathogens
Host-Pathogen Interactions
Viral load
Research Article
Adult
Population
Biology
Microbiology
Minor Histocompatibility Antigens
Asian People
In vivo
Virology
Cytidine Deaminase
Genetics
Humans
Genetic Predisposition to Disease
education
Gene
Microbial Pathogens
Evolutionary Biology
Polymorphism
Genetic
lcsh:R
Biology and Life Sciences
HIV
Viral Replication
Viral replication
Genetic Polymorphism
HIV-1
lcsh:Q
Population Genetics
Viral Transmission and Infection
Zdroj: PLoS ONE, Vol 9, Iss 3, p e92861 (2014)
PLoS ONE
ISSN: 1932-6203
Popis: Objective The human APOBEC3 family of proteins potently restricts HIV-1 replication APOBEC3B, one of the family genes, is frequently deleted in human populations. Two previous studies reached inconsistent conclusions regarding the effects of APOBEC3B loss on HIV-1 acquisition and pathogenesis. Therefore, it was necessary to verify the effects of APOBEC3B on HIV-1 infection in vivo. Methods Intact (I) and deletion (D) polymorphisms of APOBEC3B were analyzed using PCR. The syphilis, HBV and HCV infection rates, as well as CD4+ T cell counts and viral loads were compared among three APOBEC3B genotype groups (I/I, D/I, and D/D). HIV-1 replication kinetics was assayed in vitro using primary cells derived from PBMCs. Results A total of 248 HIV-1-infected Japanese men who have sex with men (MSM) patients and 207 uninfected Japanese MSM were enrolled in this study. The genotype analysis revealed no significant differences between the APOBEC3B genotype ratios of the infected and the uninfected cohorts (p = 0.66). In addition, HIV-1 disease progression parameters were not associated with the APOBEC3B genotype. Furthermore, the PBMCs from D/D and I/I subjects exhibited comparable HIV-1 susceptibility. Conclusion Our analysis of a population-based matched cohort suggests that the antiviral mechanism of APOBEC3B plays only a negligible role in eliminating HIV-1 in vivo.
Databáze: OpenAIRE
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