Lack of association between intact/deletion polymorphisms of the APOBEC3B gene and HIV-1 risk
Autor: | Hirotaka Ode, Akifumi Takaori-Kondo, Noriyo Kaneko, Yoshiyuki Yokomaku, Seiichi Ichikawa, Junji Imamura, Mayumi Imahashi, Dai Watanabe, Makoto Utsumi, Kei Sato, Takuma Shirasaka, Wataru Sugiura, Yasumasa Iwatani, Taisuke Izumi, Kazuhiro Matsuoka, Tomoki Naoe, Takashi Masaoka, Yoshio Koyanagi |
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Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: |
Male
Viral Diseases lcsh:Medicine HIV Infections Pathogenesis Pathology and Laboratory Medicine Cohort Studies Immunodeficiency Viruses Japan Polymorphism (computer science) Genotype Medicine and Health Sciences lcsh:Science education.field_of_study Multidisciplinary virus diseases Viral Load Middle Aged Infectious Diseases Medical Microbiology Viral Pathogens Host-Pathogen Interactions Viral load Research Article Adult Population Biology Microbiology Minor Histocompatibility Antigens Asian People In vivo Virology Cytidine Deaminase Genetics Humans Genetic Predisposition to Disease education Gene Microbial Pathogens Evolutionary Biology Polymorphism Genetic lcsh:R Biology and Life Sciences HIV Viral Replication Viral replication Genetic Polymorphism HIV-1 lcsh:Q Population Genetics Viral Transmission and Infection |
Zdroj: | PLoS ONE, Vol 9, Iss 3, p e92861 (2014) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | Objective The human APOBEC3 family of proteins potently restricts HIV-1 replication APOBEC3B, one of the family genes, is frequently deleted in human populations. Two previous studies reached inconsistent conclusions regarding the effects of APOBEC3B loss on HIV-1 acquisition and pathogenesis. Therefore, it was necessary to verify the effects of APOBEC3B on HIV-1 infection in vivo. Methods Intact (I) and deletion (D) polymorphisms of APOBEC3B were analyzed using PCR. The syphilis, HBV and HCV infection rates, as well as CD4+ T cell counts and viral loads were compared among three APOBEC3B genotype groups (I/I, D/I, and D/D). HIV-1 replication kinetics was assayed in vitro using primary cells derived from PBMCs. Results A total of 248 HIV-1-infected Japanese men who have sex with men (MSM) patients and 207 uninfected Japanese MSM were enrolled in this study. The genotype analysis revealed no significant differences between the APOBEC3B genotype ratios of the infected and the uninfected cohorts (p = 0.66). In addition, HIV-1 disease progression parameters were not associated with the APOBEC3B genotype. Furthermore, the PBMCs from D/D and I/I subjects exhibited comparable HIV-1 susceptibility. Conclusion Our analysis of a population-based matched cohort suggests that the antiviral mechanism of APOBEC3B plays only a negligible role in eliminating HIV-1 in vivo. |
Databáze: | OpenAIRE |
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