The role of extracellular polymeric substance matrix on Saccharomyces cerevisiae bioelectricity

Autor:	Graziela C. Sedenho, Giovana R. Mendes, Frank N. Crespilho, Iago A. Modenez
Rok vydání:	2021
Předmět:	biology Chemistry General Chemical Engineering Saccharomyces cerevisiae Flavoprotein biology.organism_classification Saccharomyces Redox Yeast ELETROFISIOLOGIA Electron transfer Extracellular polymeric substance Electrochemistry biology.protein Extracellular Biophysics
Zdroj:	Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP
Popis:	Saccharomyces cerevisiae is one of the first microorganism established to be able to convert chemical energy into electricity, however its extracellular electron transfer (EET) mechanism was still unclear. Here, we show how yeast strains extracellularly transfer electron to an electrode surface. We observed yeast cells adsorption on solid surfaces occurs through the extracellular polymeric substance (EPS) network, and EET process takes place by redox species mostly confined on the electrode surface. We demonstrated that a flavoprotein is present in the Saccharomyces cerevisiae´s EPS and is responsible by non-diffusive extracellular electron transfer through the EPS electroactive microenvironment. It is proposed EPS acts as a polyelectrolyte for multistep electron-transfer between flavoproteins and consequently for the Saccharomyces cerevisiae EET and bioelectricity generation. The elucidation of this mechanism combined to electrode engineering can contribute to the advance of biosystems in green energy generation, such as microbial fuels.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95bef24373fa0b1945b7460182185175 Zobrazit plný text záznamu