The labile iron pool reacts rapidly and catalytically with Peroxynitrite
| Autor: | José Carlos Toledo Junior, André Luis Condeles |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Paraquat
nitrogen dioxide Iron labile iron pool Isoindoles Iron Chelating Agents Microbiology Biochemistry Models Biological Article Catalysis Fluorescence peroxynitrite Nitric oxide chemistry.chemical_compound Mice Reaction rate constant stomatognathic system nitric oxide Organoselenium Compounds Peroxynitrous Acid Animals oxidative stress Chelation Nitric Oxide Donors Molecular Biology Aldehydes ESTRESSE OXIDATIVO Superoxide Hydrazones Fluoresceins QR1-502 Kinetics stomatognathic diseases RAW 264.7 Cells chemistry Yield (chemistry) Biophysics superoxide Oxidation-Reduction Peroxynitrite Intracellular |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Biomolecules Volume 11 Issue 9 Biomolecules, Vol 11, Iss 1331, p 1331 (2021) |
| Popis: | While investigating peroxynitrite-dependent oxidation in murine RAW 264.7 macrophage cells, we observed that removal of the Labile Iron Pool (LIP) by chelation increases the intracellular oxidation of the fluorescent indicator H2DCF, so we concluded that the LIP reacts with peroxynitrite and decreases the yield of peroxynitrite-derived oxidants. This was a paradigm-shifting finding in LIP biochemistry and raised many questions. In this follow-up study, we address fundamental properties of the interaction between the LIP and peroxynitrite by using the same cellular model and fluorescence methodology. We have identified that the reaction between the LIP and peroxynitrite has catalytic characteristics, and we have estimated that the rate constant of the reaction is in the range of 106 to 107 M−1s−1. Together, these observations suggest that the LIP represents a constitutive peroxynitrite reductase system in RAW 264.7 cells. |
| Databáze: | OpenAIRE |
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