New procedure for rapid identification of microorganisms causing urinary tract infection from urine samples by mass spectrometry (MALDI-TOF)
| Autor: | Raúl Ortiz de Lejarazu Leonardo, Miguel Ángel Bratos Pérez, Gabriel Alberto March Rosselló, Antonio Orduña Domingo, María Purificación Gutiérrez Rodríguez |
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| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Adult Aged 80 and over Male Chromatography Time Factors Adolescent Bacteria Chemistry Urinary system Microorganism Urine Middle Aged Mass spectrometry Rapid identification Matrix (chemical analysis) Clinical microbiology Young Adult Child Preschool Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Urinary Tract Infections Humans Female Child Aged |
| Zdroj: | Enfermedades infecciosas y microbiologia clinica. 33(2) |
| ISSN: | 1578-1852 |
| Popis: | Matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry is widely established as a technique in clinical microbiology laboratories for the identification of microorganisms. Using this technique, it is also possible to obtain the identification of microorganisms from untreated urine samples.In this study, a differential centrifugation protocol and a criterion for validation of the results in order to achieve microbial identification from untreated urine samples are proposed. Additionally, the sensitivity of the analytical procedure in monobacterial urine samples has been evaluated.A 90% sensitivity (confidence interval of 81.96%-94.84%) was obtained in urine samples with bacterial counts of ≥1×10(5)CFU/ml, and it was possible to improve the percentages of direct identifications from urine samples with bacterial counts of1×10(5)CFU/ml.It is concluded that the MALDI-TOF system is both fast and reliable in the identification of individual microorganisms from untreated urine samples with counts of ≥1×10(5)CFU/ml. |
| Databáze: | OpenAIRE |
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