Site-specific Substitutions Eliminate Aggregation Properties of Hemopressin
| Autor: | Benben Song, Kalyani Jambunathan, Patrick Kibler, Aaron N. Endsley, Surendra Kumar Nayak, Amit K. Galande |
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| Rok vydání: | 2015 |
| Předmět: |
Stereochemistry
Molecular Sequence Data Peptide Biochemistry Hemoglobins Mice Protein Aggregates chemistry.chemical_compound Drug Discovery Animals Humans Amino Acid Sequence Particle Size Amino acid residue Peptide sequence Pharmacology chemistry.chemical_classification Organic Chemistry Dynamic Light Scattering Peptide Fragments Hemopressin Amino acid Amino Acid Substitution chemistry Drug Design Hydrodynamics Molecular Medicine Amyloid like fibrils |
| Zdroj: | Chemical Biology & Drug Design. 86:1433-1437 |
| ISSN: | 1747-0277 |
| DOI: | 10.1111/cbdd.12610 |
| Popis: | Hemopressin is a naturally occurring and therapeutically relevant peptide with applications in hypertension, pain, addiction, and obesity. We had previously demonstrated that hemopressin converts into amyloid-like fibrils under aqueous conditions. However, the amino acid residues that modulate the aggregation propensity of hemopressin were not identified. In this study, we designed and synthesized 25 different analogs of hemopressin and analyzed their aggregation properties using the principle of dynamic light scattering. As a result, we were able to identify four conservative changes in the peptide sequence (Val(2) →DVal(2), Asn(3) →Gln(3) Leu(7) →Npg(7) and C-OH→C-NH2) that minimize aggregation propensity of hemopressin. The results indicate that hemopressin aggregation is cooperative in nature and involves contribution from multiple amino acids within the peptide chain. The analogs and the corresponding aggregation propensity data reported in this study would be useful for researchers investigating therapeutic properties of hemopressin, which have been hampered due to the tendency of hemopressin to aggregate in aqueous solutions. |
| Databáze: | OpenAIRE |
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