Determination of hair nicotine by gas chromatography–mass spectrometry
| Autor: | Syazwani Ismail, Gam Lay Harn, Razak Lajis, Che Nin Man, Rahmat Awang |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Nicotine
Clinical Biochemistry Mass spectrometry Sensitivity and Specificity Biochemistry Gas Chromatography-Mass Spectrometry Analytical Chemistry Young Adult medicine Humans Least-Squares Analysis Detection limit Chromatography Small volume Chemistry Smoking Extraction (chemistry) Salting Reproducibility of Results Cell Biology General Medicine Tobacco Smoke Pollution Smoking status Gas chromatography–mass spectrometry Hair medicine.drug |
| Zdroj: | Journal of Chromatography B. 877:339-342 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2008.12.014 |
| Popis: | Hair nicotine is a known biomarker for monitoring long-term environmental tobacco smoke (ETS) exposure and smoking status. In general, hair nicotine assay involves alkaline digestion, extraction and instrumental analysis. The gas chromatography-mass spectrometry (GC-MS) assay currently developed has shown to be of high throughput with average approximately 100 hair samples being extracted and analyzed per day. This was achieved through simplified extraction procedure and shortened GC analysis time. The extraction was improved by using small volume (0.4 mL) of organic solvent that does not require further evaporation and salting steps prior to GC-MS analysis. Furthermore, the amount of hair utilized in the extraction was very little (5 mg) while the sensitivity and selectivity of the assay is equal, if not better than other established methods. The linearity of the assay (r(2)>0.995), limit of quantitation (0.04 ng/mg hair), within- and between-assays accuracies and precisions ( |
| Databáze: | OpenAIRE |
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