Performance of a new Candida anti-mannan IgM and IgG assays in the diagnosis of candidemia
| Autor: | Mei Kang, Tingting Wang, Dongdong Li, Ying Ma, Yanming Meng, Ziwei Kuang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male lcsh:Arctic medicine. Tropical medicine Adolescent diagnosis lcsh:RC955-962 Hospitalized patients 030231 tropical medicine Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Candida infections Mannans Young Adult 03 medical and health sciences High morbidity 0302 clinical medicine Diagnosis Humans Medicine Antibodies Fungal Aged Mannan biology business.industry Candida colonization candidemia Case-control study Candidemia Middle Aged anti-mannan Immunoglobulin M Anti-mannan Case-Control Studies Immunoglobulin G Immunology biology.protein Original Article Female Antibody business |
| Zdroj: | Revista do Instituto de Medicina Tropical de São Paulo; Vol. 62 (2020); e25 Revista do Instituto de Medicina Tropical de São Paulo; v. 62 (2020); e25 Revista do Instituto de Medicina Tropical de São Paulo Instituto de Medicina Tropical (IMT) instacron:IMT Revista do Instituto de Medicina Tropical de São Paulo, Vol 62 Revista do Instituto de Medicina Tropical de São Paulo, Volume: 62, Article number: e25, Published: 11 MAY 2020 |
| ISSN: | 1678-9946 0036-4665 |
| Popis: | Candida is one of the most frequent pathogens of bloodstream infections, which is associated with high morbidity and mortality rates. Rapid immunological detection methods are essential in the early diagnosis of candidemia. Anti-mannan is one of host-derived biomarkers against cell wall components of Candida. We conducted this study to evaluate the diagnostic performance of two anti-mannan assays (IgM, IgG) for candidemia through the analysis of 40 candidemia patients, 48 participants with Candida colonization and 213 participants with neither Candida colonization nor Candida infections (13 patients with other bloodstream infections, 145 hospitalized patients and 55 healthy controls). The performance of the two assays were evaluated by calculating their sensitivity and specificity. The sensitivity ranged from 0.78 to 0.80 for the IgM assay and 0.68 to 0.75 for the IgG assay. The specificity ranged from 0.97 to 0.98 for the IgM assay and 0.91 to 0.94 for the IgG assay. The diagnostic performance of the anti-mannan IgM assay was better than that of IgG, with higher sensitivity and specificity. Combining the two assays (positive results of single or both assays are both considered as positive) could improve the sensitivity up to 0.93 (0.79-0.98) and only slightly reduce the specificity (0.93(0.89-0.95)). The anti-mannan IgM, IgG assays are rapid and cost-effective assays that may be probably useful in the diagnosis of candidemia. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|