Chronic Academic Stress Increases a Group of microRNAs in Peripheral Blood
| Autor: | Kazuhito Rokutan, Yuki Kuwano, Shizuka Kano, Kensei Nishida, Yoshiko Kamezaki, Kinuyo Fujita, Kiyoshi Masuda, Sakurako Katsuura-Kamano, Manami Honda, Yoko Akaike |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Period (gene) Physiology lcsh:Medicine Biology Protein Serine-Threonine Kinases Fibroblast growth factor Wnt4 Protein Gene expression microRNA medicine Humans Receptor Fibroblast Growth Factor Type 1 Receptor lcsh:Science Hydrocortisone Multidisciplinary Teaching lcsh:R MicroRNAs Immunology Anxiety lcsh:Q RNA extraction medicine.symptom Carrier Proteins Stress Psychological medicine.drug Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 10, p e75960 (2013) |
| ISSN: | 1932-6203 |
| Popis: | MicroRNAs (miRNAs) play key roles in regulation of cellular processes in response to changes in environment. In this study, we examined alterations in miRNA profiles in peripheral blood from 25 male medical students two months and two days before the National Examination for Medical Practitioners. Blood obtained one month after the examination were used as baseline controls. Levels of seven miRNAs (miR-16, -20b, -26b, -29a, -126, -144 and -144*) were significantly elevated during the pre-examination period in association with significant down-regulation of their target mRNAs (WNT4, CCM2, MAK, and FGFR1 mRNAs) two days before the examination. State anxiety assessed two months before the examination was positively and negatively correlated with miR-16 and its target WNT4 mRNA levels, respectively. Fold changes in miR-16 levels from two days before to one month after the examination were inversely correlated with those in WNT4 mRNA levels over the same time points. We also confirmed the interaction between miR-16 and WNT4 3′UTR in HEK293T cells overexpressing FLAG-tagged WNT4 3′UTR and miR-16. Thus, a distinct group of miRNAs in periheral blood may participate in the integrated response to chronic academic stress in healthy young men. |
| Databáze: | OpenAIRE |
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