In vitro effects of Crotalus atrox snake venom on chick and mouse neuromuscular preparations
| Autor: | Raphael Schezaro-Ramos, Stephen Hyslop, Beatriz B. Pereira, Victor Corasolla Carregari, Noroska Gabriela Salazar Mogollón, José R. Almeida, Saulo L. da Silva, Ananda T Santa Fé Miguel, Bruno Mendes |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Snake venom Physiology Health Toxicology and Mutagenesis Myotoxin Diaphragm Drug Resistance Neuromuscular Junction Paraspinal Muscles Venom In Vitro Techniques Pharmacology Toxicology complex mixtures Biochemistry Mice 03 medical and health sciences Nerve Fibers Species Specificity Crotalid Venoms medicine Animals Muscle Skeletal Crotalus atrox Neuromuscular Blockade biology Crotalus Neurotoxicity Cell Biology General Medicine Myotoxicity medicine.disease biology.organism_classification Phenotype In vitro Phrenic Nerve Spinal Nerves 030104 developmental biology Organ Specificity North America Neuromuscular blockade Neuromuscular Blocking Agents Chickens Muscle Contraction |
| Zdroj: | Repositorio Universidad Regional Amazónica Universidad Regional Amazónica instacron:IKIAM |
| ISSN: | 1532-0456 |
| DOI: | 10.1016/j.cbpc.2018.03.008 |
| Popis: | The neuromuscular effect of venoms is not a major clinical manifestation shared between rattlesnakes native to the Americas, which showed two different venom phenotypes. Taking into account this dichotomy, nerve muscle preparations from mice and chicks were used to investigate the ability of Crotalus atrox venom to induce in vitro neurotoxicity and myotoxicity. Unlike crotalic venoms of South America, low concentrations of C. atrox venom did not result in significant effects on mouse neuromuscular preparations. The venom was more active on avian nerve-muscle, showing reduction of twitch heights after 120 min of incubation with 10, 30 and 100 μg/mL of venom with diminished responses to agonists and KCl. Histological analysis highlighted that C. atrox was myotoxic in both species of experimental animals; as evidenced by degenerative events, including edematous cells, delta lesions, hypercontracted fibers and muscle necrosis, which can lead to neurotoxic action. These results provide key insights into the myotoxicity and low neurotoxicity of C. atrox in two animal models, corroborating with previous genomic and proteomic findings and would be useful for a deeper understanding of venom evolution in snakes belonging to the genus Crotalus. |
| Databáze: | OpenAIRE |
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