IMGT/HighV QUEST paradigm for T cell receptor IMGT clonotype diversity and next generation repertoire immunoprofiling
| Autor: | Vincent Corbin, Bruce E. Loveland, Marie-Paule Lefranc, Eric J. Gowans, Anthony T. Papenfuss, Paul U. Cameron, Jean-Pierre Y. Scheerlinck, Magdalena Plebanski, Michael A. Frohman, J. Douglas Freeman, Patrice Duroux, Scott R. Burrows, Véronique Giudicelli, John J. Miles, Shuo Li, Eltaf Alamyar |
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| Přispěvatelé: | Department of Microbiolgy and Immunology, School of Biomedical Sciences [Melbourne], Faculty of Medicine, Dentistry and Health Sciences [Melbourne], University of Melbourne-University of Melbourne-Faculty of Medicine, Dentistry and Health Sciences [Melbourne], University of Melbourne-University of Melbourne, Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Receptors Antigen T-Cell alpha-beta T cell Receptors Antigen T-Cell General Physics and Astronomy Immunogenetics Computational biology Biology Article General Biochemistry Genetics and Molecular Biology DNA sequencing 03 medical and health sciences 0302 clinical medicine T-Cell Receptor Beta medicine Humans Alleles ComputingMilieux_MISCELLANEOUS 030304 developmental biology Clonal diversity Genetics Internet 0303 health sciences [SDV.GEN]Life Sciences [q-bio]/Genetics Multidisciplinary Repertoire T-cell receptor Haplotype Computational Biology Genetic Variation General Chemistry Middle Aged Flow Cytometry Clone Cells 3. Good health medicine.anatomical_structure Haplotypes 030215 immunology |
| Zdroj: | Nature Communications Nature Communications, Nature Publishing Group, 2013, 4, pp.2333. ⟨10.1038/ncomms3333⟩ |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/ncomms3333⟩ |
| Popis: | T cell repertoire diversity and clonotype follow-up in vaccination, cancer, infectious and immune diseases represent a major challenge owing to the enormous complexity of the data generated. Here we describe a next generation methodology, which combines 5′RACE PCR, 454 sequencing and, for analysis, IMGT, the international ImMunoGeneTics information system (IMGT), IMGT/HighV-QUEST web portal and IMGT-ONTOLOGY concepts. The approach is validated in a human case study of T cell receptor beta (TRB) repertoire, by chronologically tracking the effects of influenza vaccination on conventional and regulatory T cell subpopulations. The IMGT/HighV-QUEST paradigm defines standards for genotype/haplotype analysis and characterization of IMGT clonotypes for clonal diversity and expression and achieves a degree of resolution for next generation sequencing verifiable by the user at the sequence level, while providing a normalized reference immunoprofile for human TRB. Dynamic changes in T cell repertoire underlie immune responses during infection, allergy, autoimmunity and cancer. Here, Li et al. present a workflow for high throughput sequencing and analysis of T cell receptor sequences, and use it to monitor the T cell response to influenza vaccination in a human patient. |
| Databáze: | OpenAIRE |
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