Analysis of LSD in human body fluids and hair samples applying ImmunElute columns
| Autor: | Jürgen Prof. Dr. Becker, J. Röhrich, S. Zörntlein |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Detection limit
Adult Male Chromatography Adolescent Chemistry Illicit Drugs Sonication Hair analysis Extraction (chemistry) Urine High-performance liquid chromatography Chromatography Affinity Pathology and Forensic Medicine Body Fluids Substance Abuse Detection Lysergic Acid Diethylamide Affinity chromatography Humans Gas chromatography–mass spectrometry Law Chromatography High Pressure Liquid Hair |
| Zdroj: | Forensic science international. 107(1-3) |
| ISSN: | 0379-0738 |
| Popis: | Immunoaffinity extraction units (LSD ImmunElute) are commercially available for the analysis of lysergic acid diethylamide (LSD) in urine. The ImmunElute resin contains immobilized monoclonal antibodies to LSD. We applied the ImmunElute procedure to serum and also to human hair samples. For hair analysis the samples were first extracted with methanol under sonication. The extracts were then purified using the ImmunElute resin. LSD analysis was carried out with HPLC and fluorescence detection. The immunoaffinity extraction provides highly purified extracts for chromatographic analysis. The limit of detection (signal-to-noise ratio = 3) has been determined to be < 50 pg regardless of which sample material was used. The procedure was applied to authentic hair samples from drug abusers (n = 11). One of these samples tested positive with an amount of 110 pg LSD in 112 mg extracted hair corresponding to a concentration of 1 pg/mg. |
| Databáze: | OpenAIRE |
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