Effect of mineral trioxide aggregate on cytokine production by peritoneal macrophages
| Autor: | Leda Quercia Vieira, D. L. Vargas, Antônio Paulino Ribeiro Sobrinho, Taia Maria Berto Rezende, Fabiano Pereira Cardoso |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Necrosis Cell Survival medicine.medical_treatment Macrophage-activating factor ved/biology.organism_classification_rank.species Mice Inbred Strains Microbiology Root Canal Filling Materials Interferon-gamma Mice medicine Macrophage Animals Aluminum Compounds General Dentistry Cells Cultured Innate immune system biology Fusobacterium nucleatum ved/biology Chemistry Interleukin-12 Subunit p40 Peptostreptococcus Tumor Necrosis Factor-alpha Silicates Peptostreptococcus anaerobius Interleukin Oxides Calcium Compounds biology.organism_classification Interleukin-12 Interleukin-10 Mice Inbred C57BL Drug Combinations Protein Subunits Cytokine Macrophages Peritoneal Female medicine.symptom |
| Zdroj: | International endodontic journal. 38(12) |
| ISSN: | 0143-2885 |
| Popis: | Aim To test the effect of two commercial brands of grey mineral trioxide aggregate (ProRoot® and MTA-Ângelus®) on cytokine production by M1 and M2 inflammatory macrophages. Methodology M1 (from C57BL/6 mice) and M2 peritoneal inflammatory macrophages (from C57BL/6 IL12p40−/− mice) were obtained and cultured in vitro in the presence of MTA. The cellular viability and the production of tumour necrosis factor-α, interleukin (IL)-12 and IL-10 in response to stimulation with interferon-γ and Fusobacterium nucleatum or Peptostreptococcus anaerobius were evaluated. Data were analysed by Mann–Whitney, Kruskal–Wallis and anova tests. Results The cements did not interfere with cellular viability or with cytokine production by either type of macrophage. However, M2 macrophages produced higher levels of IL-10 when stimulated with F. nucleatum than M1 macrophages (P |
| Databáze: | OpenAIRE |
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