Role of collagenase in mediating in vitro alveolar epithelial wound repair
| Autor: | Gillian Murphy, Chantal Lafuma, Stéphane Galiacy, Valérie Laurent, Daniel Isabey, Jelena Gavrilovic, Michael A. Matthay, Christine Clerici, Emmanuelle Planus, Marie-Pia d'Ortho |
|---|---|
| Přispěvatelé: | Galiacy, Stephane, Physiopathologie et Thérapeutiques Respiratoires, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10, School of Biological Sciences, University of East Anglia [Norwich] (UEA), Laboratoire de Physiologie, UFR de Santé, Université Paris 13 (UP13), Service de Physiologie-Exploration Fonctionnelles, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), This study has been supported by Institut National de la Santé et de la Recherche Médicale (INSERM, France, contrat no. 4M106C Intercommission no. 1), by Legs Poix (Chancellerie des Universités, Paris, France), by Medical Research Council (UK), and by NIH grant no. HL51854 (USA). |
| Rok vydání: | 1999 |
| Předmět: |
MESH: Pulmonary Alveoli
MESH: Rats Alveolar Epithelium MESH: Collagenases [SDV.BC]Life Sciences [q-bio]/Cellular Biology Lung injury Biology Epithelium Cell Line MESH: Cell Adhesion Cell Movement MESH: Collagen Matrix Metalloproteinase 13 MESH: Matrix Metalloproteinase 13 Cell Adhesion MESH: Cytoskeleton medicine Animals Humans MESH: Animals Collagenases Cell adhesion [SDV.BC] Life Sciences [q-bio]/Cellular Biology MESH: Cell Movement Cells Cultured Cytoskeleton A549 cell Wound Healing MESH: Humans Cell migration Cell Biology respiratory system Rats MESH: Cell Line Cell biology Pulmonary Alveoli MESH: Matrix Metalloproteinase 1 MESH: Wound Healing MESH: Epithelium Immunology Collagenase Collagen Matrix Metalloproteinase 1 Wound healing Type I collagen MESH: Cells Cultured medicine.drug |
| Zdroj: | Europe PubMed Central Scopus-Elsevier Journal of Cell Science Journal of Cell Science, Company of Biologists, 1999, 112 ( Pt 2), pp.243-52 EMMANUELLE PLANUS |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.112.2.243 |
| Popis: | International audience; Type II pneumocytes are essential for repair of the injured alveolar epithelium. The effect of two MMP collagenases, MMP-1 and MMP-13 on alveolar epithelial repair was studied in vitro. The A549 alveolar epithelial cell line and primary rat alveolar epithelial cell cultures were used. Cell adhesion and cell migration were measured with and without exogenous MMP-1. Wound healing of a cell monolayer of rat alveolar epithelial cell after a mechanical injury was evaluated by time lapse video analysis. Cell adhesion on type I collagen, as well as cytoskeleton stiffness, was decreased in the presence of exogenous collagenases. A similar decrease was observed when cell adhesion was tested on collagen that was first incubated with MMP-1 (versus control on intact collagen). Cell migration on type I collagen was promoted by collagenases. Wound healing of an alveolar epithelial cell monolayer was enhanced in the presence of exogenous collagenases. Our results suggest that collagenases could modulate the repair process by decreasing cell adhesion and cell stiffness, and by increasing cell migration on type I collagen. Collagen degradation could modify cell adhesion sites and collagen degradation peptides could induce alveolar type II pneumocyte migration. New insights regarding alveolar epithelial cell migration are particularly relevant to investigate early events during alveolar epithelial repair following lung injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|