Fast Retrograde Access to Projection Neuron Circuits Underlying Vocal Learning in Songbirds
| Autor: | Daniel Normen Düring, Chihiro Mori, Benjamin F. Grewe, Roman Boehringer, Richard H. R. Hahnloser, Benjamin Ehret, Melanie Rauch, Mariana Diales da Rocha, Stefan Gerber, Falk Dittrich, Shouwen Ma, Robert Kasper, Kazuo Okanoya, Ryosuke O. Tachibana, Jean-Charles Paterna, Manfred Gahr |
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| Přispěvatelé: | University of Zurich, Düring, Daniel N |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Genetics and Molecular Biology AAV viral vector retrograde labeling dopaminergic projections brainbow GCaMP birdsong songbird circuit tracing Projection neuron General Biochemistry Genetics and Molecular Biology Article Songbirds 03 medical and health sciences Mice 0302 clinical medicine 1300 General Biochemistry Genetics and Molecular Biology medicine Biological neural network Brainbow Animals 10194 Institute of Neuroinformatics Neurons biology biology.organism_classification 3. Good health Structure and function Songbird 030104 developmental biology medicine.anatomical_structure nervous system General Biochemistry 570 Life sciences ISLE Center for the Interdisciplinary Study of Language Evolution Vocal learning Neuron Vocalization Animal Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Cell Reports, 33 (6) Cell reports |
| Popis: | SUMMARY Understanding the structure and function of neural circuits underlying speech and language is a vital step toward better treatments for diseases of these systems. Songbirds, among the few animal orders that share with humans the ability to learn vocalizations from a conspecific, have provided many insights into the neural mechanisms of vocal development. However, research into vocal learning circuits has been hindered by a lack of tools for rapid genetic targeting of specific neuron populations to meet the quick pace of developmental learning. Here, we present a viral tool that enables fast and efficient retrograde access to projection neuron populations. In zebra finches, Bengalese finches, canaries, and mice, we demonstrate fast retrograde labeling of cortical or dopaminergic neurons. We further demonstrate the suitability of our construct for detailed morphological analysis, for in vivo imaging of calcium activity, and for multi-color brainbow labeling. In Brief Düring et al. describe a fast and efficient viral vector to dissect structure and function of neural circuits underlying learned vocalizations in songbirds. The AAV variant provides retrograde access to projection neuron circuits, including dopaminergic pathways in songbirds and additionally in mice, and allows for retrograde calcium imaging and multispectral brainbow labeling. Graphical Abstract |
| Databáze: | OpenAIRE |
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