Extending the classification of bacterial transcription factors beyond the helix-turn-helix motif as an alternative approach to discover new cis/trans relationships
| Autor: | Matthias Merzbacher, Maximilian Schlicht, Harald Nothaft, Bernard Joris, Sébastien Rigali, Paul A. Hoskisson, Fritz Titgemeyer |
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| Rok vydání: | 2004 |
| Předmět: |
In silico
Helix-turn-helix Biology Response Elements Genome Regulon Acetylglucosamine Bacterial Proteins Bacterial transcription Consensus Sequence Genetics Consensus sequence Gene Helix-Turn-Helix Motifs Binding Sites Bacteria Streptomyces coelicolor Phosphotransferases Computational Biology Articles biology.organism_classification Streptomyces bacteria Genome Bacterial Software Transcription Factors |
| Zdroj: | Nucleic acids research. 32(11) |
| ISSN: | 1362-4962 |
| Popis: | Transcription factors (TFs) of bacterial helix-turn-helix superfamilies exhibit different effector-binding domains (EBDs) fused to a DNA-binding domain with a common feature. In a previous study of the GntR superfamily, we demonstrated that classifying members into subfamilies according to the EBD heterogeneity highlighted unsuspected and accurate TF-binding site signatures. In this work, we present how such in silico analysis can provide prediction tools to discover new cis/trans relationships. The TF-binding site consensus of the HutC/GntR subfamily was used to (i) predict target sites within the Streptomyces coelicolor genome, (ii) discover a new HutC/GntR regulon and (iii) discover its specific TF. By scanning the S.coelicolor genome we identified a presumed new HutC regulon that comprises genes of the phosphotransferase system (PTS) specific for the uptake of N-acetylglucosamine (PTS(Nag)). A weight matrix was derived from the compilation of the predicted cis-acting elements upstream of each gene of the presumed regulon. Under the assumption that TFs are often subject to autoregulation, we used this matrix to scan the upstream region of the 24 HutC-like members of S.coelicolor. orf SCO5231 (dasR) was selected as the best candidate according to the high score of a 16 bp sequence identified in its upstream region. Our prediction that DasR regulates the PTS(Nag) regulon was confirmed by in vivo and in vitro experiments. In conclusion, our in silico approach permitted to highlight the specific TF of a regulon out of the 673 orfs annotated as 'regulatory proteins' within the genome of S.coelicolor. |
| Databáze: | OpenAIRE |
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