Simple and rapid analysis of tocilizumab using HPLC‐fluorescence detection method
| Autor: | Seitaro Kamiya, Makoto Takada, Kenichiro Nakashima, Tsutomu Kabashima, Yoshihito Ohba |
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| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Chromatography 010401 analytical chemistry Biophysics Trimethylamine 02 engineering and technology Antibodies Monoclonal Humanized 021001 nanoscience & nanotechnology 01 natural sciences High-performance liquid chromatography Fluorescence Subclass 0104 chemical sciences Amino acid Arthritis Rheumatoid chemistry.chemical_compound Tocilizumab chemistry Chemistry (miscellaneous) Humans Surface charge 0210 nano-technology Triethylamine Chromatography High Pressure Liquid |
| Zdroj: | Luminescence. 34:347-352 |
| ISSN: | 1522-7243 1522-7235 |
| DOI: | 10.1002/bio.3615 |
| Popis: | We developed a novel assay using high-performance liquid chromatography (HPLC) with fluorescence detection for the determination of tocilizumab (TCZ), after it has undergone a facile and rapid pretreatment. TCZ belongs to the same subclass as IgG1 (Immunoglobulin G subclass 1), and we could separate TCZ from IgG1 without antigen-antibody reactions, with the novel detection method. The separation of these antibodies was achieved by pretreatment with an organic solvent containing a base, such as trimethylamine and triethylamine. The effect of these bases on the separation of TCZ is related to the hydrophobicity of the base rather than the electrostatic charge. The results indicated that the surface charge of antibodies changed because of the structural change, even though the difference in the amino acid sequences of the antibodies was very low. Our method is available for the separation of the antibody subclasses, and it would be useful to assay TCZ in blood. |
| Databáze: | OpenAIRE |
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