Molecular characterisation of the tammar wallaby (Macropus eugenii) CD3 epsilon chain cDNA
| Autor: | Elizabeth M. Deane, Gavan A. Harrison, Julie M. Old |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
DNA
Complementary CD3 Complex Molecular Sequence Data Immunology Receptors Antigen T-Cell Peptide Tammar wallaby Complementary DNA Animals Humans Coding region Amino Acid Sequence Phosphorylation Molecular Biology Peptide sequence Conserved Sequence Macropus Marsupial Macropodidae chemistry.chemical_classification Binding Sites Base Sequence Sequence Homology Amino Acid biology biology.organism_classification Molecular biology chemistry |
| Zdroj: | Molecular Immunology. 38:359-364 |
| ISSN: | 0161-5890 |
| DOI: | 10.1016/s0161-5890(01)00072-4 |
| Popis: | The cDNA encoding the epsilon chain of the tammar wallaby CD3 complex (CD3epsilon) was isolated by PCR. This is the first CD3 component to be cloned in a marsupial. The tammar wallaby cDNA coding region was 61.7 and 63.0% identical to the human and mouse cDNA coding sequences, respectively. Similarly, the predicted amino acid sequence was 56.5 and 52.9% identical to the human and mouse sequences. When compared with other known CD3epsilon peptide sequences, the most conserved region of the tammar wallaby CD3epsilon chain peptide was the cytoplasmic domain and the least conserved was the extracellular portion. Phylogenetic reconstruction based on the deduced amino acid sequence placed the tammar wallaby sequence in its expected position outside of all the eutherian mammals. |
| Databáze: | OpenAIRE |
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