Structural reorganization of the fungal endoplasmic reticulum upon induction of mycotoxin biosynthesis
| Autor: | Karen Broz, Samuel O. Purvine, Marike Johanne Boenisch, Lanelle R. Connolly, Carrie D. Nicora, H. C. Kistler, William B. Chrisler, Scott E. Baker, Michael Freitag |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Trichothecene Secondary Metabolism Biology Endoplasmic Reticulum 01 natural sciences Article Fungal Proteins 03 medical and health sciences Fusarium Microscopy Electron Transmission Live cell imaging Organelle Cellular localization Triticum Plant Diseases Multidisciplinary Endoplasmic reticulum Compartmentalization (psychology) Mycotoxins In vitro 3. Good health Cell biology Biosynthetic Pathways Metabolic pathway Luminescent Proteins 030104 developmental biology Biochemistry Microscopy Fluorescence Trichothecenes 010606 plant biology & botany |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Compartmentalization of metabolic pathways to particular organelles is a hallmark of eukaryotic cells. Knowledge of the development of organelles and attendant pathways under different metabolic states has been advanced by live cell imaging and organelle specific analysis. Nevertheless, relatively few studies have addressed the cellular localization of pathways for synthesis of fungal secondary metabolites, despite their importance as bioactive compounds with significance to medicine and agriculture. When triggered to produce sesquiterpene (trichothecene) mycotoxins, the endoplasmic reticulum (ER) of the phytopathogenic fungus Fusarium graminearum is reorganized both in vitro and in planta. Trichothecene biosynthetic enzymes accumulate in organized smooth ER with pronounced expansion at perinuclear- and peripheral positions. Fluorescence tagged trichothecene biosynthetic proteins co-localize with the modified ER as confirmed by co-fluorescence and co-purification with known ER proteins. We hypothesize that changes to the fungal ER represent a conserved process in specialized eukaryotic cells such as in mammalian hepatocytes and B-cells. |
| Databáze: | OpenAIRE |
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