Lipid peroxidation and HSP72/73 expression in rat following cadmium chloride administration: Interactions of magnesium supplementation
| Autor: | Fatma Ghorbel, Fatma Makni-Ayadi, Christian Vincent, Abelfettah El-Feki, Manel Boujelben, Françoise Croute, Fadhel Guermazi |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty Antioxidant Thiobarbituric acid medicine.medical_treatment HSP72 Heat-Shock Proteins Cadmium chloride Kidney Toxicology medicine.disease_cause Thiobarbituric Acid Reactive Substances Pathology and Forensic Medicine Lipid peroxidation chemistry.chemical_compound Cadmium Chloride Internal medicine Testis medicine TBARS Animals Drug Interactions Magnesium Rats Wistar Dose-Response Relationship Drug Chemistry Kidney metabolism Cell Biology General Medicine Carcinogens Environmental Rats medicine.anatomical_structure Endocrinology Liver Lipid Peroxidation Oxidative stress |
| Zdroj: | Experimental and Toxicologic Pathology. 57:437-443 |
| ISSN: | 0940-2993 |
| DOI: | 10.1016/j.etp.2006.02.012 |
| Popis: | Objective : to determine whether magnesium (Mg) supplementation could have a protective effect against the cadmium (Cd)-induced oxidative stress in liver, kidneys and testes of adult male rats. Stress was evaluated by measuring lipid peroxidation by thiobarbituric acid reactive substances (TBARS) and the heat shock protein (HSP) 72/73 expression. CdCl 2 injections (2.5 mg/day/kg body weight) for 10 days resulted in a time dependent increase of Cd accumulation in liver, kidney and testes, the highest levels being found in liver (400 μg/g dried tissue). At the same time, an increase of lipid peroxidation was observed. The effect was maximal at day 1 of Cd treatment in liver and testes, and later (day 5) in kidney. Then, Cd-induced lipid peroxidation decreased, suggesting the activation of antioxidant defense mechanisms. Injections of Mg SO 4 (300–600 mg/day/kg body weight) reduced in a dose-dependent manner Cd-induced lipid peroxidation in liver and kidney as well as the accumulation of Cd in liver, kidney and testes. In testes, a protective effect of Mg was found only during the early phase of Cd-poisoning. On days 5 and 10, lipid peroxidation was even increased as compared to controls. In liver and testes only the constitutive HSP73 was detected whereas in kidney both HSP73 and the inducible HSP72 were expressed. HSP72/73 expression was not significantly increased by Cd and HSP73 was even lowered in kidney, probably due to the strong dose used. These results were not modified by Mg injections. Conclusion : Mg supplementation can reduce Cd accumulation in organs and lipid peroxidation related to Cd administration. |
| Databáze: | OpenAIRE |
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